The monocarboxylate transporter 8 (MCT8) is a member of the major facilitator superfamily (MFS). These membrane-spanning proteins facilitate translocation of a variety of substrates, MCT8 specifically transports iodothyronines. Mutations in MCT8 are the underlying cause of severe X-linked psychomotor retardation. At the molecular level, such mutations led to deficiencies in substrate translocation due to reduced cell-surface expression, impaired substrate binding, or decreased substrate translocation capabilities. However, the causal relationships between genotypes, molecular features of mutated MCT8, and patient characteristics have not yet been comprehensively deciphered. We investigated the relationship between pathogenic mutants of MCT8 and their capacity to form dimers (presumably oligomeric structures) as a potential regulatory parameter of the transport function of MCT8. Fourteen pathogenic variants of MCT8 were investigated in vitro with respect to their capacity to form oligomers. Particular mutations close to the substrate translocation channel (S194F, A224T, L434W, and R445C) were found to inhibit dimerization of MCT8. This finding is in contrast to those for other transporters or transmembrane proteins, in which substitutions predominantly at the outer-surface inhibit oligomerization. Moreover, specific mutations of MCT8 located in transmembrane helix 2 (del230F, V235M, and ins236V) increased the capacity of MCT8 variants to dimerize. We analyzed the localization of MCT8 dimers in a cellular context, demonstrating differences in MCT8 dimer formation and distribution. In summary, our results add a new link between the functions (substrate transport) and protein organization (dimerization) of MCT8, and might be of relevance for other members of the MFS. Finally, the findings are discussed in relationship to functional data combined with structural–mechanistical insights into MCT8.
Members of the major facilitator superfamily (MFS) are membrane-spanning proteins that facilitate the translocation of diverse substrates including sugars, ions, amino acids, and many other molecules between the cytoplasm and the extracellular region (Law et al. 2008). The transporters can be found in all living species (for an overview of classification and comprehensive information, please refer to
MCT8 translocates iodothyronines (Friesema et al. 2003, Kinne et al. 2010). These molecules, acting as hormones, play multi-faceted and major roles in vertebrate growth and metabolism, and are also of great importance for neuronal cell growth and development (Friesema et al. 2006, 2010, 2012, Visser et al. 2008, James et al. 2009, Bernal 2011). A vast number of pathogenic mutations of MCT8 have been identified (reviewed in Visser et al. (2011)), comprising insertions, deletions, single side-chain substitutions, and splice site variants (Friesema et al. 2010). The affected patients primarily suffer from mental retardation, severe muscle hypotonia, and, to a greater extent, are unable to speak or walk (Schwartz et al. 2005). However, there may be individual differences in the observed phenotypes (
One of the potentially modulated properties of MCT8 arising from a mutation is oligomeric organization, which may have an influence on the functionality of this transporter. Results from previous studies have indicated (Biebermann et al. 2005) that MCT8 occurs as dimers comprising two interacting protomers, or, presumably, as higher order complexes such as tetramers (Visser et al. 2009). In support of this, other members of the MFS have also been reported to form oligomers, including the glycine transporter (Bartholomaus et al. 2008), organic cation transporter 1 (Keller et al. 2011), γ-amino butyric acid transporter 1 (Korkhov et al. 2004), multidrug-transporter EmrD (Yin et al. 2006), dopamine transporter (Hastrup et al. 2001), and serotonin transporter (Kilic & Rudnick 2000). Higher-order complexes can be functionally relevant such as those found for amphetamine transporters, for which influx and efflux occurs via separate but interacting transporter protomers (Seidel et al. 2005).
The primary goal of the current study was to examine pathogenic mutations of MCT8 pertaining to their influence on the capacity of MCT8 to form dimeric or oligomeric constellations. For this purpose, we tested 14 known pathogenic variants with partial or complete loss-of-function with regard to their capacity to form dimeric complexes in comparison with WT. Mutations were distributed over the entire transmembrane region of MCT8. Indeed, mutations were identified that alter the dimerization state of MCT8, with unexpected implications for the structural prerequisites of transporter interactions and regulation.
Material and methods
For plasmid constructs, the expression vector pcDNA3 was used containing the human MCT8 WT cDNA or MCT8 sequences with the mutations: ins189I (insertion of isoleucine between positions 188 and 189), S194F, A224V, del230F (deletion of phenylalanine 230), ins236V (insertion of valine between positions 235 and 236), V235M, R271H, L434W, L471P, G558D, and L568P. The point mutations A224T, R445C, and L512P were introduced into the cDNA of MCT8 using the QuikChange Site-Directed Mutagenesis protocol (Agilent Technologies, La Jolla, CA, USA) and confirmed by direct sequencing. In addition, a hemagglutinin-tag (YPYDVPDYA, N-HA) at the amino-terminal end or a FLAG-tag (DYKDDDDK, C-FLAG) at the carboxy-terminal end of human MCT8 WT, as well as of the mutants, was inserted.
For the bimolecular fluorescence complementation assay, fragments of yellow fluorescent protein (YFP) were fused at the C-terminal end to the MCT8 WT and MCT8 mutant cDNA (A224T and del230F) in accordance with the method described by Nyfeler et al. (2005).
The pcDNA3 (MCT8 and mutants) constructs were kindly provided by Dr Ulrich Schweizer (Rheinische Friedrich-Wilhelms-Universität Bonn, Germany), and the pcDNA3 (fragment-YFP1 and fragment-YFP2) constructs were provided by Dr Hans-Peter Hauri (University of Basel, Switzerland).
Transiently transfected COS-7 cells (an African green monkey kidney epithelial cell line), cultured in DMEM (Biochrom AG, Berlin, Germany), supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin, 100 μg/ml streptomycin, and 2 mM l-glutamine were used in dimerization studies of the pathogenic MCT8 mutants.
Investigation of dimerization of MCT8 via sandwich ELISA
To investigate dimerization of MCT8, a sandwich ELISA was performed with N-HA and C-FLAG-tagged WT MCT8 and mutants as described previously (Biebermann et al. 2005). MCT8 mutants were compared with the co-transfection of N-HA-WT and C-FLAG-WT (positive control). N-HA-WT transfection alone was used as a negative control (CTRL). In brief, 600 000 COS-7 cells were seeded in 6 cm dishes and transiently transfected with 3 μg DNA using 6 μl Metafectene. At 72-h post-transfection, cells were washed, harvested, and solubilized in 200 μl lysis buffer containing 10 mM Tris–HCl of pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol (DTT), 1% sodium-deoxycholate, 1% Nonidet P-40, and 0.2 mM phenyl-methyl-sulfonyl fluoride overnight at 4 °C. Ninety-six-well plates were coated with monoclonal anti-FLAG-M2 antibody (10 μg/ml in 0.15 M sodium tetraborate, pH 8; Sigma–Aldrich) overnight at 4 °C. After blocking with 10% FBS in DMEM for 1 h at room temperature, the plates were washed twice with 1× PBS containing 0.05% Tween-20 (PBS-T). Following removal of cell debris, the cell lysates were incubated in the FLAG-antibody-coated 96-well plates in triplicates overnight at 4 °C. The plates were washed four times with PBS-T, followed by the addition of a biotin-labeled monoclonal anti-HA antibody (1 μg/ml in PBS-T; Roche) and incubation for 2 h. The plates were washed again four times with PBS-T and incubated with peroxidase-conjugated streptavidin (0.2 μg/ml in PBS-T; BioLegend, London, UK) for 1 h. After a final washing step (four times with PBS-T), H2O2 and o-phenylendiamine (2.5 mM each in 0.1 M phosphate–citrate buffer, pH 5.0) were used as substrate and chromogen. The enzyme reaction was stopped by the addition of 1 M HCl containing 0.05 M NaSO3. The color intensity was measured at 492 nm and normalized to the absorbance values at 620 nm. The readings were normalized to the protein concentrations of the cell lysates as measured with the BCA Protein Assay Reagent Kit (Thermo Fisher Scientific, Rockford, IL, USA).
It should be noted that the detection of dimers in the sandwich-ELISA approach is limited by the fact that only the combination of N-HA with C-FLAG MCT8 can be determined. The formation of N-HA/N-HA MCT8 in addition to C-FLAG/C-FLAG MCT8 also occurs but cannot be detected.
In order to investigate the significant differences between the dimerization of the differently tagged WT as a positive control and the mutants, an unpaired two-tailed Student's t-test with Welch's correction was performed. Statistical analyses were performed using GraphPad Prism version 5.00 for Windows (GraphPad Software, San Diego, CA, USA).
Confocal microscopy of MCT8 dimers
The YFP-based protein fragment complementation assay (PCA; Nyfeler et al. 2005) was used to confirm decreased and increased dimerization of mutants of MCT8 and their trafficking pathways in expressing COS-7 cells. In this assay, YFP is split into two halves. One half is fused to one MCT8 protomer and the other half to the other MCT8 protomer. Upon dimerization, the two halves of YFP reconstitute, forming a functioning YFP which then can be detected. Fluorescent YFP was reconstituted with regard to the dimerization of MCT8–YFP1 and –YFP2 constructs, i.e. fluorescence indicated the formation of productive and stable dimers.
COS-7 cells were seeded into six-well plates (300 000 cells/well) on poly-l-lysine-coated cover glasses and transiently transfected with 4 μl Metafectene and 1.5 μg DNA (MCT8–YFP1 and MCT8–YFP2)/well. Approximately 24 h after transfection, cells were fixed with DAPI–methanol solution (1 μg DAPI/ml) for 10 min at 37 °C and mounted with Vectashield Hard Set (Vector Laboratories, Burlingame, CA, USA) on microscope slides. The cells were observed under a Zeiss LSM 510 META laser scanning microscope equipped with argon and helium-neon lasers (Carl Zeiss GmbH, Jena, Germany). The optical sections were obtained with a pinhole setting of 1 Airy unit at a resolution of 1024×1024 pixels, and the images were further analyzed using LSM 510 Software, release 3.2 (Carl Zeiss GmbH).
Molecular homology modeling of MCT8 and a potential mode of substrate binding
A new structural human MCT8 homology model was generated by using the same general procedure as described previously (Kleinau et al. 2011), with a few significant modifications. Generally, this model is based on a structural transporter template in an ‘inward-facing’ conformation, namely the crystal structure of the glycerol-3-phosphate transporter (GlpT) (PDB code 1PW4 (Huang et al. 2003)). In contrast to our previous homology models, we refined the orientations of the loop regions and side-chains by extended molecular dynamic simulation. In addition, the structural template used indicated that helix 12 of MCT8 might be constituted by residues between Ala553 and Asp589. In accordance with the crystal structures of other members of the MFS, such as lactose permease (PDB code 1PV6 (Abramson et al. 2003)), or the recently solved ‘outward-facing’ and substrate bound conformation of a glucose transporter (PDB code 4GBZ (Sun et al. 2012)), this helix is shorter and is regular up to the position corresponding to MCT8 amino acid position Pro572, followed by a loop region that continues with a short helix. Moreover, this small helical section interacts with further transporter regions, as has been observed for other crystal structures, and these might constitute stabilizing interactions (Sun et al. 2012). Therefore the MCT8 helix 12 was constructed in accordance with these structural features and terminates with a regular helix conformation at Pro572, whereby adjacent residues are allocated close to the intracellular loops.
By fixing the backbone atoms, molecular dynamics (4 ns) were subjected to the flexible amino acid side-chains of the model (Sybyl-X2.0, Certara, Princeton, NJ, USA). The entire system was then minimized by fixing the backbone atoms of the transmembrane helices (TMHs) until convergence at a termination gradient of 0.05 kcal/mol×Å. This procedure was repeated thrice. Finally, the model was minimized without constraints using the AMBER 7.0 force field. Structure images were produced using the PyMOL Molecular Graphics System, version 1.3 (Schrödinger, LLC, New York, NY, USA).
The resulting structural model was utilized to demonstrate the positions of mutations, visualize the substrate translocation channel (Fig. 1A, B and C), and also docking of T3 into MCT8 (Fig. 1D). Substrate docking (details of the docking procedure are described in the
The intention of this study was to unravel the question of whether naturally occurring mutations in MCT8 might have an effect on formation of MCT8 dimers. Fourteen known naturally occurring mutations, which have been identified in affected patients, were chosen. These mutations are distributed over the entire transporter structure and they are mostly characterized by a single-side-chain substitution. These 14 variants constitute the main fraction of known naturally occurring single-side-chain substitutions. Exon deletions or stop-codon variants were not explored. For seven of these variants of MCT8 (ins189I, A224V, R271H, L471P, L512P, G558D, and L568P), dimer assembly was identical to that of WT MCT8 dimers as determined by sandwich ELISA (Table 1 and Fig. 2). However, modifications of the dimerization ability were detected for seven variants of MCT8 (S194F, A224T, del230F, V235M, ins236V, L434W, and R445C).
Spatial localization of MCT8 mutations in the protein, and effects on dimerization. The mutations analyzed here are located in different helices, or transitions between helices and loops. Besides the regular position number (starting from the start-codon position 1) for each mutation, the number in accordance with the unifying MCT-group numbering scheme is also provided (Kinne et al. 2010), in order to enable comparison between different members of the MCT group. This position identifier scheme uses a highly conserved residue in each TMH as a common reference for all members of the MCT family. The first number is related to the particular helix, the second number after the dot determines the specific position in relation to the conserved residue. For example, the highly conserved proline in TMH2 is defined as 2.50 and the highly conserved glycine from TMH3 is defined as 3.50. The nomenclature follows the standard conventions of the Human Genome Variation Society (HGVS)
|Mutation (nomenclature of HGVS)||Abbreviation used in this study||Dimerization compared with WT||Structural localization||Unifying number|
Four mutants are characterized by diminished (−) capacity to homo-dimerize compared with the WT. Three mutations in TMH2 lead to increased dimerization (+). ‘=’ Indicates equal to WT. TMH, transmembrane helix; ECL, extracellular loop; ICL, intracellular loop.
Substrate uptake of WT and mutant variants of MCT8 in COS-7 cells
Extensive functional in vitro characterization of MCT8 mutants revealed that the results obtained might vary depending on the cellular system used (Jansen et al. 2005, 2008, Kinne et al. 2009, Capri et al. 2013, Kersseboom et al. 2013). This therefore plays a decisive role in why a precise established relationship does not exist between the genotype, molecular characteristics of MCT8 variants (localization, transport routes), and the severity of patient phenotypes (Visser et al. 2011) (summarized in
However, we analyzed COS-7 cells for their suitability for the functional characterization of MCT8 mutants with respect to T3 uptake. The T3 transport function of six particular pathogenic variants of MCT8 (S194F, A224T, del230F, V235M, L471P, and L568P) was confirmed to be reduced in comparison with WT (
Specific naturally occurring mutations inhibit dimerization of MCT8
Modifications of the dimeric state of specific MCT8 mutants were detected: S194F in TMH1, A224T in TMH2, L434W (ECL4/TMH8) and R445C in TMH8 were observed to result in a reduced level of dimerization by 30–60% in comparison with WT mutants (Table 1 and Fig. 3). The A224T mutation led to a reduction in dimerization capability to form oligomers, by approximately 65%, while the R445C, S194F, and L434W mutations resulted in 53, 45, and 36% reductions in dimerization capability in comparison with WT, respectively, as assessed by sandwich ELISA.
Interestingly, at position Ala224, two amino acid substitutions altered dimeric formation differentially. The A224V variant formed homo-dimeric complexes similarly to the WT, whereas in mutant A224T, the structure of MCT8 was mostly monomeric.
The WT positions of the four dimerization altering amino acids S194, A224, L434, and R445 were mapped onto the MCT8 model and revealed a spatial localization directly at or in close proximity to the transport channel cavity (‘inward-facing’ conformation; Figs 1 and 4). This finding is in contrast to the hypothesis that only substitutions at the protein surface disrupt the protomer–protomer contacts.
Mutations in TMH2 led to an increased capacity for dimerization
Surprisingly, we detected increased dimerization capabilities in the variants of MCT8 with the del230F, V235M, and ins236V mutations (Table 1 and Fig. 3). The increase in dimers detectable by sandwich ELISA amounted up to 150–175% when compared with WT values, i.e. del230F reached 148%, V235M 151%, and ins236V 174%.
According to our structural MCT8 model, amino acid substitutions, deletions, or insertions, causing increased MCT8 dimerization (Table 1 and Fig. 4) are located exclusively in TMH2 of the WT protein and thus outside of the transport channel cavity. Insertions or deletions modify the orientation of this specific helical section close to the intracellular site. It is noteworthy that the side-chain at position 235 is without any intramolecular interaction partner in this monomeric MCT8 model and substitution of V235M, therefore, does not alter any side-chain interactions. However, all three mutations indicate that this structural section should be of great importance in the formation of higher-order complexes.
Investigation of WT and mutant MCT8 by bimolecular complementation and confocal laser scanning microscopy analysis
To verify data obtained with the sandwich-ELISA approach, we choose one MCT8 variant from the group with increased dimerization (del230F) and one characterized by decreased dimerization (A224T). Dimerization of WT and of A224T or del230F MCT8 proteins was analyzed using a bimolecular fluorescence complementation assay. In this assay, fluorescence is only detectable upon dimer formation.
The cells expressing MCT8 WT dimers (Fig. 5A, B and C) appeared evenly distributed and firmly adherent to the cover glasses, while fluorescence was detectable in reticular structures reminiscent of the endoplasmic reticulum, in vesicles (arrows), and at the cell surface (arrowheads). In addition, approximately 50% of cells expressing WT dimers exhibited small foci of aggregated, strongly fluorescent protein distributed throughout the cytoplasm.
Likewise, cells forming dimers of the del230F (Fig. 5D, E and F) mutant were adherent and disseminated on the substratum displaying fluorescence in reticular and vesicular structures (arrows), as well as at the cell surface (arrowheads). In comparison with WT dimers, del230F-mutant expressing cells contained numerous and smaller foci of fluorescent dimeric or oligomeric proteins, which were often observed to have a bead-on-a-string appearance, which is expected for transport vesicles in transit from the Golgi apparatus to the cell surface (Fig. 5D). These findings are in accordance with the sandwich-ELISA data, thus indicating an enhanced dimerization capability compared with WT MCT8.
In stark contrast, cells expressing the A224T dimeric proteins (Fig. 5G, H and I) were less evenly distributed on the substratum, with a curved shape and fewer cellular extensions in comparison with WT dimers and del230F dimer-expressing cells. Moreover, COS-7 cells expressing the A224T variant of MCT8 were characterized by the presence of prominent foci containing fluorescent protein which accumulated in the peri-nuclear regions, which are characteristic of the so-called aggresomes that are typically formed as a consequence of protein misfolding and overload of the ER-associated degradation machinery. Therefore, the formation of A224T dimers at the cell surface of expressing COS-7 cells is less frequent than in cells expressing WT MCT8 or the del230F variant. Results obtained using confocal laser scanning microscopy thus indicate a potential association with results obtained with the sandwich-ELISA method; however, we cannot rule out the possibility that aggresomes identified by laser scanning microscopy could be detected using the sandwich-ELISA approach.
In the current study, pathogenic mutations leading to partial monomerization of MCT8 were identified (S194F, A224T, L434W, and R445C). Specific mutants (del230F, V235M, and ins236V) with an increased level of dimerization, and oligomerization, were also found (Fig. 3). Interestingly, the R445C (Groeneweg et al. 2014) and ins236V (Kersseboom et al. 2013) variants of MCT8 have been examined in COS cells, using approaches which included analyses of their dimerization capacity and cellular distribution. In accordance with our findings, the western blotting data presented in previous studies (Kersseboom et al. 2013, Groeneweg et al. 2014) might also indicate increased (ins236V) or decreased (R445C) dimerization of these MCT8 variants.
Transporter oligomers and structure–function relationships
It is known from studies of G-protein-coupled receptors (GPCRs) that an equilibrium may exist between monomers and oligomers (Lambert 2010, Teichmann et al. 2012, Calebiro et al. 2013), implying that protomer–protomer interfaces are flexible or transient. Amino acid mutations can shift this equilibrium toward a monomeric or more oligomeric state if they modify the interface(s) between the protomers.
It remains unknown how this protomer–protomer interaction is constituted for MCT8. Cysteine residues that are potentially disulfide bridged do not participate in the structure of putative MCT8 protomer interface(s) in oligomers (Visser et al. 2009), as has been reported, for example, for GPCRs (Berthouze et al. 2007) and other members of the MFS (Hastrup et al. 2003, Henriksen et al. 2005). Moreover, it has been demonstrated that the amino acids from positions 267 to 360 (putative TMH4–6) are not involved in MCT8 protomer contacts (Visser et al. 2009). However, several contact regions for membrane-spanning transporter oligomers have already been reported (oligomerization is inhibited by mutation): the bacterial Ca2+/H+ antiporter (Ridilla et al. 2012) – contacts at TMH2 and TMH6; the Na+/H+ antiporter NhaA (Gerchman et al. 2001) – loop transition on the cytoplasmatic side of helix 8; the dopamine transporter (Hastrup et al. 2003) – at the extracellular end of TMH6; the γ-amino-butyric acid transporter 1 – at TMH2 (Korkhov et al. 2004); and the glycine transporter 2 (GlyT2) – extracellular transition of TMH6. Very recently, TMH2 has been identified as a crucial dimerization interface of the bacterial sweet transporter (Xu et al. 2014).
It is of note that, taking these insights into dimer-constitution, different interfaces of MFS members, and our findings on MCT8 under consideration, it might be also feasible that truncated or mutated (non-functional) variants of MCT8 interact with other membrane proteins such as further members of the MFS family. This should theoretically lead also to dominant-negative effects on interacting WT proteins.
Notably, the protomer–protomer contacts for those transporters are spatially located at the protein surface in order to establish intermolecular contacts. In contrast, in this current study, the identified mutations associated with inhibition of dimerization are located close to the putative substrate-translocation channel in the center of MCT8. In addition, mutations in TMH2 of MCT8 are surface-directed and increase the capacity for oligomerization. A number of explanations could be put forward for these findings. In order to adequately address this, the thus far proposed molecular transport mechanisms at MCT8 will be briefly described: for transporter monomers, it is assumed that substrate binding triggers the transition between ‘outward-facing’ and ‘inward-facing’ conformations by a so-called ‘rocker-switch’ mechanism (rocking back and forth around a central axis), whereby the structure of the transporter undergoes global structural rearrangements (Huang et al. 2003, Yin et al. 2006, Law et al. 2008, Sun et al. 2012). Following substrate binding, the two transporter domains A and B (Fig. 1) move closer, either intracellularly or extracellularly, respectively. This process is supported by flexibility in specific helices caused by irregular helical conformations. The global movement (rocking back and forth) is triggered by substrate binding between the domains that weakens or/and increases specific intramolecular (inter-helical) interactions. As a consequence, mutations in the transport channel (with sensitivity for substrate binding) are also likely to have a dramatic effect on the transporter conformation, because they are localized at predestined positions to initiate global structural shifts. These conformational shifts induced in the transport channel of protomers could also modify the oligomer organization by indirectly altering the interfaces between the (modified) protomers.
But how should this relate to the function of the transporters? It is known that transporters can function by distinct mechanisms, such as i) uniporters, which transport only one type of substrate (substrate-gradient regulated); ii) antiporters, which transport two or more substrates but in opposite directions across the membrane, and iii) symporters, which translocate two or more substrates in the same direction simultaneously. Hence, an oligomeric transporter organization could be linked with the counter-transport of substrate(s), particularly the regulation of influx and efflux that might occur through separated but coupled protomers (Seidel et al. 2005). In a potential protomer–protomer constellation for coupled substrate influx/efflux, the two transporter molecules could have different conformations that mutually modify one another during substrate transport. As a consequence, particular mutations in the transport channel that modify the transporter–protomer conformation might also disturb the functional interplay between protomers in an oligomeric constellation.
TMH2 is a key player in the function of MCT8
Our results highlight helix 2 as a potential key player in the function of MCT8. This hypothesis is supported by the finding of mutations that increase dimerization at a specific section of TMH2 close to the intracellular site. From their location and functional data (Figs 3 and 4), we can conclude that this helical part most probably weakens the oligomeric interface – ‘destabilization’ – and that mutations (and their resulting structural modifications) reverse this negative effect on oligomerization.
Moreover, the alanine-to-threonine substitution at position 224 in the more central part of the protein leads to oligomer dissociation. The A224V single substitution differs from the A224T mutant and does not alter the oligomeric state (Figs 2 and 3). This indicates a fine-tuned molecular mechanism at this WT residue. Valine is hydrophobic and bulkier than alanine and will, therefore, lead to repulsion in this tightly packed vicinity close to the substrate-translocation region (inhibiting substrate transport directly or indirectly). In contrast, threonine is hydrophilic and smaller than valine, but might interact via H-bonds with further side-chains in close proximity (like Tyr419) or with the helical backbone. In conclusion, a constraint (H-bond) at position 224 might inhibit oligomerization, whereas the flexibility of valine in a hydrophobic cage retains the capacity for oligomerization.
The results of this study indicate that there is a relationship between oligomerization and functional parameters of MCT8, but the question on an obligatory functional need for dimerization of MCT8 remains to be answered. Furthermore, on the cellular level mutant variants of MCT8 might interact differently with other ancillary proteins for proper localization at the plasma membrane and transport function. Interaction with the regulator PTTG-binding factor (PBF) has been previously demonstrated to be associated with a reduction in expression of MCT8 at the cell surface (Smith et al. 2012). Finally, our data put emphasis on the general question surrounding substrate-induced or constitutive oligomerization versus monomerization (oligomer dissociation) in members of the MFS, which has not yet been elucidated.
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Declaration of interest
The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.
This work was supported by the Deutsche Forschungsgemeinschaft (DFG), projects BI 893/6-3, BI893/5-1, KL 2334/2-2, Ki 1751/1-1, MO 1018/2-1, FU 356/7-1 and 8-1, and BR 1308/11-1.
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(J Fischer and G Kleinau contributed equally to this work)