Expression and purification of biologically active porcine follicle-stimulating hormone in insect cells bearing a baculovirus vector
in Journal of Molecular EndocrinologySearch for other papers by Y Kato in
Current site
Google Scholar
PubMed
Search for other papers by I Sato in
Current site
Google Scholar
PubMed
Search for other papers by T Ihara in
Current site
Google Scholar
PubMed
Search for other papers by K Tomizawa in
Current site
Google Scholar
PubMed
Search for other papers by J Mori in
Current site
Google Scholar
PubMed
Search for other papers by M Geshi in
Current site
Google Scholar
PubMed
Search for other papers by T Nagai in
Current site
Google Scholar
PubMed
Search for other papers by K Okuda in
Current site
Google Scholar
PubMed
Search for other papers by T Kato in
Current site
Google Scholar
PubMed
Search for other papers by S Ueda in
Current site
Google Scholar
PubMed
Biologically active recombinant porcine FSH (rec-pFSH) free from the cognate pituitary glycoprotein hormone LH was produced. It was synthesized by a baculovirus vector-insect cell system using two cDNAs encoding the glycoprotein alpha and FSH beta subunits. Its antigenicity was the same as that of pFSH prepared from the pituitary. Glycosylation of rec-pFSH was shown by tunicamycin treatment but the molecular mass of each subunit was lower than that of pituitary-derived FSH, because of the absence of trimming of terminal sugars in insect cells. Rec-pFSH was secreted into the culture medium at about 1 mg/l and purified in six fractions, because of the heterogeneity of the sugar group, by S-Sepharose and concanavalin A-Sepharose column chromatography. The biological activity of rec-pFSH was examined by measuring its effect on progesterone secretion from porcine granulosa cells and germinal vesicle breakdown (GVBD) of porcine oocytes. It showed adequate activity with respect to progesterone secretion, although some fractions rich in the sugar group showed lower activity than that of pituitary-derived FSH. It exhibited higher GVBD activity than that of pituitary-derived FSH at concentrations as low as 1 ng/ml. These results demonstrate that the baculovirus vector-insect cell system can provide biologically active rec-pFSH.
Journal of Molecular Endocrinology is committed to supporting researchers in demonstrating the impact of their articles published in the journal.
The two types of article metrics we measure are (i) more traditional full-text views and pdf downloads, and (ii) Altmetric data, which shows the wider impact of articles in a range of non-traditional sources, such as social media.
More information is on the Reasons to publish page.
| Sept 2018 onwards | Past Year | Past 30 Days | |
|---|---|---|---|
| Full Text Views | 330 | 184 | 38 |
| PDF Downloads | 155 | 60 | 11 |
Online ISSN: 1479-6813
Print ISSN: 0952-5041
Strengthening biomedical communities
to advance science and health
CONTACT US
Bioscientifica Ltd | Starling House | 1600 Bristol Parkway North | Bristol BS34 8YU | UK
Bioscientifica Ltd | Registered in England no 3190519