that this space could be occupied by combining ChIP with droplet digital PCR (ddPCR) ( Hindson et al. 2011 ). Following purification, ChIP DNA is added to ddPCR mastermix with primers directed at positive or negative regions. The reaction mixture is
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Ann Louise Hunter, Natasha Narang, Matthew Baxter, David W Ray, and Toryn M Poolman
A Pestka, B Toth, C Kuhn, S Hofmann, I Wiest, G Wypior, K Friese, and U Jeschke
, Glostrup, Denmark) for 6 min, counterstained with hemalaun for 30 s, washed in tap water, and cover-slipped. The cells were examined with a Leitz Diaplan light microscope (Leitz, Wetzlar, Germany) and a digital camera system (JVC, Victor Company of Japan
M Essand, S Vikman, J Grawé, L Gedda, C Hellberg, K Oberg, T H Totterman, and V Giandomenico
metastases, obtained at different times, while the rest of the primary and metastasis specimens were from independent patients. The study was approved by the local ethics committee (Dnr Ups 02–077). Reverse transcription (RT)–PCR
Masanori Ito, Tomohiko Urano, Hisahiko Hiroi, Mikio Momoeda, Mayuko Saito, Yumi Hosokawa, Ryo Tsutsumi, Fumiko Zenri, Minako Koizumi, Hanako Nakae, Kuniko Horie-Inoue, Tomoyuki Fujii, Tetsu Yano, Shiro Kozuma, Satoshi Inoue, and Yuji Taketani
ESCs ( Gellersen & Brosens 2003 ). The decidualization of ESCs was confirmed by the expression of IGFBP1 and prolactin ( PRL ) mRNA extracted from ESC by quantitative RT-PCR (qRT-PCR) analysis. The ESCs were treated with 0.5 mM 8-Br-cAMP and/or 10 −6
M Blumenstein, J A Keelan, J M Bowen-Shauver, and M D Mitchell
was reported ( Marvin et al. 1999 ) according to grades 0 to 4 for degree of infiltration of the gestational membranes, and + or – for each of the fetal membranes ( Salafia et al. 1989 ). Qualitative RT-PCR First
Su M Hlaing, Leah A Garcia, Jaime R Contreras, Keith C Norris, Monica G Ferrini, and Jorge N Artaza
microscope, Leica DMLB, coupled to a Spot RT digital camera. RT 2 profiler PCR array analysis of cell cycle and WNT-related target genes Total cellular RNA was isolated using Trizol Reagent (Invitrogen) from H9c2 cells treated with or without 1,25-D 3 (100
Agua Sobrino, Pilar J Oviedo, Susana Novella, Andrés Laguna-Fernandez, Carlos Bueno, Miguel Angel García-Pérez, Juan J Tarín, Antonio Cano, and Carlos Hermenegildo
antibodies (from Sigma), followed with nitroblue tetrazolium/5-bromo-4-chloro-3-indolyl phosphate, p -toluidine salt color development reaction. Blots were digitalized using a Gelprinter PLUS (TDI, Madrid, Spain), and the densities of spots were analyzed
Dong-Jae Jun, Kyung-Yoon Na, Wanil Kim, Dongoh Kwak, Eun-Jeong Kwon, Jong Hyuk Yoon, Kyungmoo Yea, Hyeongji Lee, Jaeyoon Kim, Pann-Gill Suh, Sung Ho Ryu, and Kyong-Tai Kim
2 days. Each medium was changed every 2 days. RNA extraction and real-time quantitative reverse transcription-PCR Total RNA was extracted from differentiated adipocytes using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. For
Thomas P Meehan, Barry G Harmon, Megan E Overcast, Kristine K Yu, Sally A Camper, David Puett, and Prema Narayan
. Materials and methods Generation of transgenic mice YHR and D556H rLHR-myc were cloned under the control of the 6 kb mouse inhibin α-subunit promoter (Fig. 1A ). PCR products were generated from YHR in pcDNA3 (3317 bp) and D556H
Ann Lo, Weiming Zheng, Yimei Gong, John R Crochet, and Lisa M Halvorson
.1(+) (Invitrogen). RNA extraction, reverse transcription, and PCRs Total RNA was prepared from adult mouse or rat pituitaries or from cultured LβT2 cells using TRI Reagent (Ambion) according to the manufacturer's instructions. Total RNA samples were DNase treated
