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Martina Holubová, Lucie Hrubá, Barbora Neprašová, Zuzana Majerčíková, Zdeňka Lacinová, Jaroslav Kuneš, Lenka Maletínská and Blanka Železná

, Brno, Czech Republic). All measurements were performed according to the manufacturer’s instructions. FOSB immunohistochemistry After the treatment, 4 mice from each group were deeply anesthetized with sodium pentobarbital (50 mg

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Edson F Nogueira, Claudia A Vargas, Mélissa Otis, Nicole Gallo-Payet, Wendy B Bollag and William E Rainey

, FOSB, NR4A3, and EGR4. Figure 1 Microarray analysis of human H295R cells after treatment for 1 h with 10 nM Ang-II versus basal (panel A). B shows confirmation with qPCR of gene up-regulation of the top five genes, which act as transcription factors

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J. M. Stephens, M. D. Butts and P. H. Pekala


3T3-L1 preadipocytes differentiate into cells having the biochemical properties of adipocytes; tumour necrosis factor-α (TNF) attenuates this process. Inhibition of differentiation by this cytokine, thought to be mediated at the level of transcription, has been investigated by examining the accumulation of mRNA for six transcription factors and three diversely regulated genes during the first 24 h of the differentiation process. Upon induction of differentiation, a rapid and major accumulation of c-fos and jun-B mRNA, which returned to near basal levels within 4–6 h, was observed. In contrast, c-jun mRNA, although rapidly expressed at the induction of differentiation, remained at relatively constant levels throughout the time-course. Exposure of the cells to 5 nm TNF potentiated the accumulation of all three mRNAs but most significantly that of c-jun (12-fold), which remained elevated for at least 24 h after treatment. In control differentiating cells, krox-20 and fos-B were expressed transiently from 30 min to 2 h, while fra-1 mRNA accumulated over an extended period of 1 to 8 h. Again, TNF enhanced the accumulation of these mRNAs. Accumulation of mRNA for C/EBP, a transcription factor proposed to control the expression of genes involved in the terminally differentiated state, was attenuated after exposure of the cells to TNF. Interleukin-6 (IL-6) mRNA was expressed briefly (30 min to 2 h) and again transiently (at 8 h after induction of differentiation). TNF treatment markedly enhanced accumulation of IL-6 message. We propose that an increased cellular content of one or more transcription factors or the suppression of C/EBP may be responsible for the attenuation of differentiation induced by exposure of the cells to TNF.

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Edson F Nogueira, Yewei Xing, Claudia A V Morris and William E Rainey

NR4A1), NURR-1 (also named NR4A2), and NOR-1 (also named NR4A3)), members of the AP-1 complex (FOS, FOSB, and JUNB), the early growth response gene 1 (EGR1), and the activating transcription factor 3 (ATF3; Nogueira et al . 2007 ). These findings

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Pulak R Manna and Douglas M Stocco

). Within this region, we have identified a motif (5′-TGACTGA-3′) that recognizes both the cAMP response element (CRE) and JUN (JUN and FOS) family proteins ( Manna et al . 2002 a , 2004 ). JUN (JUN, JUNB, and JUND) and FOS (FOS, FOSB, FOSL1, and FOSL2

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Jean-Guy LeHoux and Andrée Lefebvre

is a family of dimeric basic region-leucine zipper proteins that recognize TPA response elements (TRE) and CRE. AP-1 dimers are composed of Jun family protein (c-Jun, JunB, JunD) homodimers, or Jun heterodimers with Fos (c-Fos, FosB, Fra-1, Fra-2), or

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Ankita Agrawal and Alison Gartland

) , Liu et al . (2008) , Okumura et al . (2008) , Panupinthu et al . (2008) , Gavala et al . (2010) , Genetos et al . (2011) and Grol et al . (2012) Induction of transcription factor FosB/AP-1 Sustained proton efflux and

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Dagmara Rusinek, Sylwia Szpak-Ulczok and Barbara Jarzab

proapoptotic factors to be characteristic of FTC, among them being NR4A1 and A3 , encoding orphan nuclear receptors involved in the regulation of proliferation and apoptosis, as well as FOSB and JUN . Loss of apoptosis and growth arrest factors occurred

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Ana Paula Lepique, Miriam S Moraes, Kátia M Rocha, Claudia B Eichler, Glaucia N M Hajj, Telma T Schwindt and Hugo A Armelin

synthesis initiation by 8–9 h ( Armelin et al. 1996 , Lotfi et al. 1997 , Lotfi & Armelin 2001 , Rocha et al. 2003 , Schwindt et al. 2003 ). ACTH, to some extent, resembles FGF2 by inducing transcription of c-fos, fosB and junB genes ( Kimura et

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David Aguinaga, Mireia Casanovas, Rafael Rivas-Santisteban, Irene Reyes-Resina, Gemma Navarro and Rafael Franco

. Intracerebroventricular administration of disrupting peptides, induces, sustains, accelerates and exacerbates the incentive motivational and locomotor activating effects of cocaine in a self-administration paradigm. The blocking peptides were also able to increase ∆FosB