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D T Furuya, A C Poletto, H S Freitas and U F Machado

, corresponding to the −83 to −62 bp κB site of Slc2a4 gene 5′-flanking sequence, Slc2a4 –NF-κB (−134), GGGTGG GGG CG T GG CC TTTTGG corresponding to −134 to −113 bp κB site of Slc2a4 gene 5′-flanking sequence, and Slc2a4 -sterol regulatory element-binding

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Horng-Yih Ou, Hung-Tsung Wu, Feng-Hwa Lu, Yu-Chu Su, Hao-Chang Hung, Jin-Shang Wu, Yi-Ching Yang, Chao-Liang Wu and Chih-Jen Chang

, USA). Farnesyl thiosalicylic acid (FTS) and the WST-8 proliferation assay kit were purchased from Cayman Chemicals (Ann Arbor, MI, USA). The following primary antibodies were used: sterol regulatory element-binding protein 1 (SREBP1; Novus, Littleton

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Hyeon Young Park, Hye Suk Kang and Seung-Soon Im

regulated by sterol regulatory element-binding proteins (SREBPs) ( Brown & Goldstein 1997 ). SREBPs are synthesized in a precursor form. For activation, the complex of SREBP with SREBP cleavage-activating protein (SCAP) dissociates from insulin-induced genes

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Thomas Ohnesorg, Brigitte Keller, Martin Hrabé de Angelis and Jerzy Adamski

roles in the regulation of steroidogenic genes ( Omura & Morohashi 1995 , Piao et al. 1997 , Guo et al. 2003 ). Genes of cholesterol biosynthesis and homoeostasis are mostly regulated by sterol regulatory element-binding protein 2 (SREBP-2

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Guohui Wan and King Ming Chan

376857), SLβ (AJ867249), GH (AJ937858), PRL (NM_181437), IGF1 (NM_131825), IGF2a (NM_131433), IGF2b (NM_001001815), IGF3 (EU272146), leptin (LEP, NM_001128576), sterol regulatory element binding protein 1 (SREBP1, DQ836065), SREBP2 (ENSDARG00000063438

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L Lundholm, S Moverare, KR Steffensen, M Nilsson, M Otsuki, C Ohlsson, JA Gustafsson and K Dahlman-Wright

Estrogens reduce adipose tissue mass in both humans and animals. The molecular mechanisms for this effect are, however, not well characterized. We took a gene expression profiling approach to study the direct effects of estrogen on mouse white adipose tissue (WAT). Female ovariectomized mice were treated for 10, 24 and 48 h with 17beta-estradiol or vehicle. RNA was extracted from gonadal fat and hybridized to Affymetrix MG-U74Av2 arrays. 17beta-Estradiol was shown to decrease mRNA expression of liver X receptor (LXR) alpha after 10 h of treatment compared with the vehicle control. The expression of several LXRalpha target genes, such as sterol regulatory element-binding protein 1c, apolipoprotein E, phospholipid transfer protein, ATP-binding cassette A1 and ATP-binding cassette G1, was similarly decreased. We furthermore identified a 1.5 kb LXRalpha promoter fragment that is negatively regulated by estrogen. Several genes involved in lipogenesis and lipolysis were identified as novel targets that could mediate estrogenic effects on adipose tissue. Finally, we show that ERalpha is the main estrogen receptor expressed in mouse white adipose tissue (WAT) with mRNA levels several hundred times higher than those of ERbeta mRNA.

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M Egea, I Metón and I V Baanante

F 1999 Sterol regulatory element binding protein-1c is a major mediator of insulin action on the hepatic expression of glucokinase and lipogenesis-related genes. PNAS 96 12737 –12742. Graham FL & Van der Eb AJ

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R Buettner, K G Parhofer, M Woenckhaus, C E Wrede, L A Kunz-Schughart, J Schölmerich and L C Bollheimer

, Raghow R & Elam MB 2005 Insulin activates the rat sterol-regulatory-element-binding protein 1c (SREBP-1c) promoter through the combinatorial actions of SREBP, LXR, Sp-1 and NF-Y cis -acting elements. Biochemical Journal 385 207 –216

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Lingxia Pang, Lianghui You, Chenbo Ji, Chunmei Shi, Ling Chen, Lei Yang, Fangyan Huang, Yahui Zhou, Jun Zhang, Xiaohui Chen and Xirong Guo

as Krüppel-like factors (KLFs) ( Mori et al . 2005 , Oishi et al . 2005 ), early growth response 20 (KROX20) ( Chen et al . 2005 ), and sterol regulatory element-binding protein 1c (SREBP1c) ( Kim et al . 1998 ), which are activated by adipogenic

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Hanna K Nyblom, Ernest Sargsyan and Peter Bergsten

al . 2008 ), which is less harmful for β-cell function than when exposed to saturated fatty acids ( Moffitt et al . 2005 ). It remains to be determined to what extent these proportions are altered when sterol regulatory element binding protein (SREBP