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Jan Wilde, Maria Erdmann, Michael Mertens, Gabriele Eiselt, and Martin Schmidt

2001 ). For site-directed mutagenesis of putative transcription factor-binding sites, the QuikChange II XL Kit (Stratagene, La Jolla, CA, USA) was used according to the manufacturer's recommendations. The sequences of all the constructs were verified by

Open access

Feng Zhang, Qi Xiong, Hu Tao, Yang Liu, Nian Zhang, Xiao-Feng Li, Xiao-Jun Suo, Qian-Ping Yang, and Ming-Xin Chen

-expression of C/EBPα downregulated ACOX1 luciferase activity. (B) The schematic diagram of site-directed mutagenesis in the predicted C/EBPα binding sites in the ACOX1 promoter. (C and D) A series of mutants of three C/EBPα binding sites were constructed and

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Kun Chen, Ji-Dan Zhou, Feng Zhang, Fang Zhang, Rui-Rui Zhang, Meng-Si Zhan, Xiao-Yin Tang, Bing Deng, Ming-Gang Lei, and Yuan-Zhu Xiong

:// ) ( Fig. 2 A). Further analysis of a putative C/EBPβ binding site suggested that it is highly conserved in human, mouse and pig ( Fig. 2 B). Site-directed mutagenesis was performed using a WT pGL3-P5 construct as a template, aiming to functionally

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Thomas Ohnesorg, Brigitte Keller, Martin Hrabé de Angelis, and Jerzy Adamski

, The Netherlands) was used. All constructs were confirmed by sequencing. Site-directed mutagenesis Mutant constructs of TF-binding sites were generated using the QuikChange Site-Directed Mutagenesis (Stratagene

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See-Tong Pang, Wen-Chi Hsieh, Cheng-Keng Chuang, Chun-Hsiang Chao, Wen-Hui Weng, and Horng-Heng Juang

with 1:1000 diluted monoclonal TXNIP antiserum (JY2; MBL International Corp., Woburn, MA, USA) or 1:1000 diluted anti β-actin antiserum (C11, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Reporter vector constructs and site-directed mutagenesis The

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Nikolaos Volakakis, Michal Malewicz, Banafsheh Kadkhodai, Thomas Perlmann, and Gerard Benoit

-activators and utilizes an alternative LBD region for transcriptional activation. In this study, we have used structural modeling to identify an alternative co-activator interaction surface in the Nurr1 LBD. Combined with site-directed mutagenesis, these studies

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Anne-Marie O’Carroll, Stephen J Lolait, and Gillian M Howell

.3) buffer and visualized by autoradiography by exposure to Kodak XAR film at −80 °C with intensifying screens. Site-directed mutagenesis The rat APJR 5′-flanking region 1 kb construct, bp −966 to −7 subcloned into the pGL3

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Raquel Tobar-Rubin, Dahlia Sultan, Daniela Janevska, Kyle Turcic, Julie Carroll, Laura Ooms, and Robin Pals-Rylaarsdam

(Guthrie cDNA Resource Center) was subjected to site-directed mutagenesis using the Stratagene QuickChange II XL site-directed mutagenesis kit. Mutagenic primers introduced or eliminated unique restriction sites (silent mutations) into the cDNA encoding

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Neil W Salter, Sudharsana R Ande, Hoa K Nguyen, B L Grégoire Nyomba, and Suresh Mishra

Technologies (Rockville, MD, USA). Naturally occurring TG2 mutants and the transamidation-activity dead Cys277Ala-TG2 mutant (as a negative control for transamidation function of TG2) were made using a site-directed mutagenesis kit ( Ande et al . 2009 a , b

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George G J M Kuiper, Willem Klootwijk, and Theo J Visser

). After the D1 cDNA cloning ( Berry et al. 1991 a ), site-directed mutagenesis and deletion analysis have identified several functional regions of the D1 protein ( Berry et al. 1991 b , 1992 , Toyoda et al. 1995 a , b , 1997 , Leonard et al