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Lucia Kořínková, Martina Holubová, Barbora Neprašová, Lucie Hrubá, Veronika Pražienková, Michal Bencze, Martin Haluzík, Jaroslav Kuneš, Lenka Maletínská, and Blanka Železná

fa/fa rats lacking functional leptin receptor signaling, negligible PrRP mRNA expression was detected ( Ellacott et al . 2002 ). Leptin deficiency in ob/ob mice causes obesity based on hyperphagia and decreased energy expenditure; in addition

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N Hoggard, D V Rayner, S L Johnston, and J R Speakman

food intake, when administered i.p. to lean C57BL/6 mice. This effect was reproduced in leptin-deficient ob/ob mice, suggesting [Nle 4 , d -Phe 7 ]-α MSH acts independently of leptin. i.p. administration of AgRP did not differ in its effects from i

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N Hoggard, M Cruickshank, K M Moar, P Barrett, S Bashir, and J D B Miller

diabetic obese ob/ob mice, 24 h fasted C57BL6 mice and in the 3T3-L1 adipocyte cell line. Methods Animals Wild-type and obese ob/ob male mice on the C57BL6 background (all ∼11 weeks old) were purchased from Harlan UK Ltd (Bicester, Oxon, UK). Animals

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Hongjie Zhang, Jing Li, Xiangying Liang, Yun Luo, Ke Zen, and Chen-Yu Zhang

mice, wild-type littermates, normal C57BL/6J mice, and ob/ob mice. We also used NCI-H716 cells, a well-characterized human L-cell model, to further verify the effect of UCP2 on secretion of GLP1. Materials and methods Animals and sample preparation

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Yoo Mee Kim, Juan Ji An, Yong-Jun Jin, Yumie Rhee, Bong Soo Cha, Hyun Chul Lee, and Sung-Kil Lim

-old C57BL/6J ob / ob mice (Central Lab. Animal Inc., Seoul, South Korea), and 6-week-old Otsuka Long–Evans Tokushima fatty (OLETF) rats (Tokushima Research Institute Otsuka Pharmaceutical Co. Ltd, Tokushima, Japan) were obtained. Six-week-old C57BL/6J

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N Hoggard, S Bashir, M Cruickshank, J D B Miller, and J R Speakman

on the expression of NMB in adipose tissue, 24 obese ( ob / ob ) mice and 24 lean (+/?) mice were used. Half the mice in each group, with ad libitum access to food and water, were injected i.p. once only with leptin (2 mg/kg body weight in saline

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N T Lam, S D Covey, J T Lewis, S Oosman, T Webber, E C Hsu, A T Cheung, and T J Kieffer

i.p. injection for 2 days. The lower dosage of leptin in this study relative to the study with the C57BL/6 mice was because of the hypersensitivity of ob/ob mice to leptin. We have previously found that this dose of leptin does not affect body

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Bo Li, Zhiguo Zhang, Huizhi Zhang, Kai Quan, Yan Lu, Dongsheng Cai, and Guang Ning

dysfunction and the pathogenesis of hepatic steatosis. Materials and methods Mice experiments and human liver tissues Eight to ten weeks old, male C57BL/6 lean mice, db/db mice and ob/ob mice ( Coleman & Hummel 1973 , Chen et al . 1996 ) that originated

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Feng Wang, Xianfeng Zhang, Jiqiu Wang, Maopei Chen, Nengguang Fan, Qinyun Ma, Ruixin Liu, Rui Wang, Xiaoying Li, Mingyao Liu, and Guang Ning

plasma lipids in ob/ob mice and Lgr4 – L eptin double-mutant mice. Plasma was obtained from male mice fed ad libitum at the indicated times. The time reflects circadian hours, with the lights on at ZT0 and off at ZT12. Total triglyceride (A) and

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S-M Luoh, F Di Marco, N Levin, M Armanini, M-H Xie, C Nelson, G L Bennett, M Williams, S A Spencer, A Gurney, and F J de Sauvage


Leptin, the product of the ob gene, is a hormone secreted by fat cells which is primarily involved in the regulation of body weight. We have generated a leptin immunoadhesin (leptin-IgG) which was more potent than leptin alone at reducing body weight and food intake when injected into ob/ob mice. This molecule was used to identify high affinity binding sites on human embryonic 293 kidney cells and subsequently to isolate a cDNA encoding the leptin receptor from this cell line by expression cloning. This receptor corresponds to the short form of the recently isolated leptin receptor. Analysis of the expression pattern of the two forms of receptor by Northern blot, in situ hybridization and quantitative PCR showed that the receptor is expressed in most tissues but that the long form is prevalent in the hypothalamus.