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Introduction Analysis of protein–DNA interactions, using chromatin immunoprecipitation (ChIP), has provided much new information in the understanding of signalling pathways key to cancer, metabolism and development. For example, the

then transferred to a nylon membrane. The dried nylon was scanned with GE ImageQuant LAS4000 mini (GEHealthcare, Shanghai, China). Chromatin immunoprecipitation assay Chromatin immunoprecipitation assay (ChIP) was performed using EZ-ChIP Chromatin

. 1997 , Tremblay et al . 1999 ). The chromatin immunoprecipitation (ChIP) assay is a powerful tool to study protein:DNA interactions in their native chromatin context ( Collas & Dahl 2008 ). Several recent studies using ChIP assays have explored the

, we examined binding of EGR1 to promoter sequences of potential target genes using collective chromatin immunoprecipitation (ChIP) assay followed by quantitative real-time PCR (qPCR) and demonstrated that down-regulation of EGR1 is a common regulator

is both enabled and limited by the tools available for analysis of this process. In this review, we summarize how the use of chromatin immunoprecipitation (ChIP)-based assays advanced our knowledge of nuclear hormone signaling, while also revisiting

shown using a combination of gel retardation and chromatin immunoprecipitation (ChIP) assays that the IGRP promoter, like the insulin promoter, binds Pax6, Pdx1, BETA2, E12/47, and USF ( Martin et al . 2003 , 2004 ). To complete this comparison of the

37°C. The blots were visualized using ECL plus kit (Beyotime, China) and the bands were quantified using Image J (NIH). Chromatin immunoprecipitation assay (ChIP) and ChIP followed by deep sequencing (ChIP-seq) ChIP assay was performed using

identify the GC-responsive region within its promoter region. For this purpose, we used a chromatin immunoprecipitation (ChIP) scanning assay to determine GR binding to the hDUSP1 promoter and promoter deletions coupled to a reporter gene in transient

subsequently found to bind to ∼700 gene promoters (from ∼20 000 gene promoters) using a Chromatin immunoprecipitation (ChIP)-on-chip assay. About 400 targets were identified and categorized into three major functional groups involved in cell cycle, metabolic

. aurata glud promoter. A ChIP assay was performed on S. aurata liver. The upper part of the figure shows a schematic drawing of S. aurata glud promoter, location of the PCR primers (arrows) and sequence of E-box at position −10 to −5 relative to the