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Ann Louise Hunter, Natasha Narang, Matthew Baxter, David W Ray, and Toryn M Poolman

Introduction Analysis of protein–DNA interactions, using chromatin immunoprecipitation (ChIP), has provided much new information in the understanding of signalling pathways key to cancer, metabolism and development. For example, the

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Kun Chen, Ji-Dan Zhou, Feng Zhang, Fang Zhang, Rui-Rui Zhang, Meng-Si Zhan, Xiao-Yin Tang, Bing Deng, Ming-Gang Lei, and Yuan-Zhu Xiong

then transferred to a nylon membrane. The dried nylon was scanned with GE ImageQuant LAS4000 mini (GEHealthcare, Shanghai, China). Chromatin immunoprecipitation assay Chromatin immunoprecipitation assay (ChIP) was performed using EZ-ChIP Chromatin

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Zoi Papoutsi, Chunyan Zhao, Milica Putnik, Jan-Åke Gustafsson, and Karin Dahlman-Wright

. 1997 , Tremblay et al . 1999 ). The chromatin immunoprecipitation (ChIP) assay is a powerful tool to study protein:DNA interactions in their native chromatin context ( Collas & Dahl 2008 ). Several recent studies using ChIP assays have explored the

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Hiroshi Ishikawa, Makio Shozu, Masahiko Okada, Mai Inukai, Bo Zhang, Keiichi Kato, Tadayuki Kasai, and Masaki Inoue

, we examined binding of EGR1 to promoter sequences of potential target genes using collective chromatin immunoprecipitation (ChIP) assay followed by quantitative real-time PCR (qPCR) and demonstrated that down-regulation of EGR1 is a common regulator

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Aurimas Vinckevicius and Debabrata Chakravarti

is both enabled and limited by the tools available for analysis of this process. In this review, we summarize how the use of chromatin immunoprecipitation (ChIP)-based assays advanced our knowledge of nuclear hormone signaling, while also revisiting

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Cyrus C Martin, Brian P Flemming, Yingda Wang, James K Oeser, and Richard M O'Brien

shown using a combination of gel retardation and chromatin immunoprecipitation (ChIP) assays that the IGRP promoter, like the insulin promoter, binds Pax6, Pdx1, BETA2, E12/47, and USF ( Martin et al . 2003 , 2004 ). To complete this comparison of the

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Xue Wen, Yao Xiong, Huimin Liu, Ting Geng, Ling Jin, Ming Zhang, Ling Ma, and Yuanzhen Zhang

37°C. The blots were visualized using ECL plus kit (Beyotime, China) and the bands were quantified using Image J (NIH). Chromatin immunoprecipitation assay (ChIP) and ChIP followed by deep sequencing (ChIP-seq) ChIP assay was performed using

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Krishan Johansson-Haque, Elanchelian Palanichamy, and Sam Okret

identify the GC-responsive region within its promoter region. For this purpose, we used a chromatin immunoprecipitation (ChIP) scanning assay to determine GR binding to the hDUSP1 promoter and promoter deletions coupled to a reporter gene in transient

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Yunguang Tong and Tamar Eigler

subsequently found to bind to ∼700 gene promoters (from ∼20 000 gene promoters) using a Chromatin immunoprecipitation (ChIP)-on-chip assay. About 400 targets were identified and categorized into three major functional groups involved in cell cycle, metabolic

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Carlos Gaspar, Jonás I Silva-Marrero, María C Salgado, Isabel V Baanante, and Isidoro Metón

. aurata glud promoter. A ChIP assay was performed on S. aurata liver. The upper part of the figure shows a schematic drawing of S. aurata glud promoter, location of the PCR primers (arrows) and sequence of E-box at position −10 to −5 relative to the