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F Al-Khafaji, M Wiltshire, D Fuhrer, G Mazziotti, M D Lewis, P J Smith and M Ludgate

means of investigating the prolonged effects of iodide on the proliferation, signal transduction and cell cycle profiles of thyroid cells expressing TSHR gain-of-function mutations. We now report our findings of these investigations

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H K Kinyamu, J Chen and T K Archer

polymerase II in Saccharomyces cerevisiae. Molecular and Cellular Biology 19 6972 –6979. Bech-Otschir D , Seeger M & Dubiel W 2002 The COP9 signalosome: at the interface between signal transduction and ubiquitin

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Ingrid Langer, Christelle Langlet and Patrick Robberecht

the basis of extensive study of the largest family of GPCRs also called the GPCR-A/rhodopsin family ( Ferguson 2001 , Kohout & Lefkowitz 2003 , Perry & Lefkowitz 2002 , Wess 1997 ). The mechanisms regulating the GPCR-B family signal transduction are

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Ray-Jade Chen, His-Chin Wu, Mu-Hsin Chang, Chao-Hung Lai, Yun-Chen Tien, Jin-Ming Hwang, Wu-Hsien Kuo, Fuu-Jen Tsai, Chang-Hai Tsai, Li-Mien Chen, Chih-Yang Huang and Chun-Hsien Chu

signaling transduction in the heart remains unclear. In a variety of cardiovascular disorders, including myocardial infarction, cardiomyocytes apoptosis in the heart has been identified as key to heart failure ( Kang & Izumo 2000 , Grazette & Rosenzweig

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L Couture, H Naharisoa, D Grebert, J-J Remy, E Pajot-Augy, V Bozon, T Haertle and R Salesse

ABSTRACT

The LH/hCG receptor is a G protein-coupled receptor with an N-terminal extracellular domain involved in hormone—receptor interaction. The recombinant porcine receptor, stably expressed in Chinese hamster ovary (CHO) cells, has the same characteristics (K d and cAMP production) as in Leydig cells. Six synthetic peptides derived from the receptor ectodomain and two polyclonal anti-peptide sera were tested in the homologous system porcine LH and porcine LH receptor. Their ability to inhibit hormone binding and signal transduction on CHO cells expressing the recombinant receptor was evaluated. Peptides 25–40 and 107–121 exhibited a high transduction inhibition as compared with hormone binding, peptides 21–36, 102–111, and 102–121 inhibited hormone binding more efficiently than signal transduction, and peptide 7–24 exhibited inhibition of both hormone binding and hormone-induced cAMP production. Immuno-globulins against peptides 21–36 and 102–111 inhibited both hormone binding and receptor activation suggesting that these sequences are located on the receptor surface.

The data suggest that multiple, discontinuous regions of the extracellular domain of porcine LH receptor are involved in hormone binding and signal transduction. Two minimum critical sequences, 21–24 and 102–107, are involved in hormone binding and vicinal segments may be implicated in signal transduction.

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Jürgen M Weiss, Heike Hüller, Stephan Polack, Michael Friedrich, Klaus Diedrich, Oliver Treeck, Georg Pfeiler and Olaf Ortmann

transduction such as calcium signaling and inositol phosphates are in part responsible for the effects of estradiol ( Ortmann et al. 1992 , 1995 ). It might well be that steroids affects late steps in GnRH signal transduction like exocytosis ( Ortmann et al

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Michelle Colomiere, Michael Permezel and Martha Lappas

, which appears to be regulated through IRS-1/Akt/PDK-1-dependent pathway. This study suggests that impaired insulin-signalling transduction disrupts glucose transport, glycogen synthesis and possibly disrupts lipid metabolism in adipose tissue

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Maciej Pietrzak and Monika Puzianowska-Kuznicka

figure represents the mean results of nine experiments, ± s.d . Discussion In this work, we showed that MCL-1, an anti-apoptotic member of the BCL-2 family, is activated by T 3 using a non-genomic mechanism that involves the PI3-K signal transduction

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H Douglas Falls, Brian D Dayton, Dennis G Fry, Christopher A Ogiela, Verlyn G Schaefer, Sevan Brodjian, Regina M Reilly, Christine A Collins and Wiweka Kaszubska

activation of GHS-R by artificial GHSs showed a variety of responses such as an increase in intracellular cAMP and free Ca 2+ levels as well as activation of PKA and PKC ( Chen 2000 ). Recently, the ghrelin-stimulated GHS-R signal transduction pathway has

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H Tabuchi, Y Furuichi and C Miyamoto

ABSTRACT

To investigate the nuclear signalling pathway induced by endothelin (ET) isopeptides, we have established permanent Chinese hamster ovary (CHO) cell lines, CHO-ETA/fos-lacZ and CHO-ETB/fos-lacZ, that produce both a c-fos-β-galactosidase fusion protein and either the type A or the type B human ET receptor. These cell lines permitted a colorimetric measurement of c-fos expression, which was induced by the signal transduction system with ET receptors and ET isopeptides. We found that the ET-1-dependent c-fos expression was so efficient that it could respond to low concentrations (even a physiological concentration) of ET-1. For example, CHO-ETA/fos-lacZ and CHO-ETB/fos-lacZ responded to ET concentrations of 5×10−9 m and 5×10−13 m respectively. Using this highly sensitive system, the H-7 sensitive protein kinase was found to be involved in signal transduction mediated by ETA, and also partly in the ETB-mediated pathway. These lines of evidence suggest that c-fos expression occurs through at least two different pathways, depending on the concentration of ET in plasma.