Search Results
You are looking at 1 - 1 of 1 items for
- Author: M Itamoto x
- Refine by access: All content x
Search for other papers by H Watanabe in
Google Scholar
PubMed
Search for other papers by A Suzuki in
Google Scholar
PubMed
Search for other papers by M Kobayashi in
Google Scholar
PubMed
Search for other papers by E Takahashi in
Google Scholar
PubMed
Search for other papers by M Itamoto in
Google Scholar
PubMed
Search for other papers by DB Lubahn in
Google Scholar
PubMed
Search for other papers by H Handa in
Google Scholar
PubMed
Search for other papers by T Iguchi in
Google Scholar
PubMed
In order to understand early events caused by estrogen in vivo, temporal uterine gene expression profiles at early stages were examined using DNA microarray analysis. Ovariectomized mice were exposed to 17beta-estradiol and the temporal mRNA expression changes of ten thousand various genes were analyzed. Clustering analysis revealed that there are at least two phases of gene activation during the period up to six hours. One involved immediate-early genes, which included certain transcription factors and growth factors as well as oncogenes. The other involved early-late genes, which included genes related to RNA and protein synthesis. In clusters of down-regulated genes, transcription factors, proteases, apoptosis and cell cycle genes were found. These hormone-inducible genes were not induced in estrogen receptor (ER) alpha knockout mice. Although expression of ERbeta is known in the uterus, these findings indicate the importance of ERalpha in the changes in gene expression in the uterus.
