Search Results

You are looking at 1 - 3 of 3 items for

  • Author: Hsien-Ming Wu x
  • Refine by access: All content x
Clear All Modify Search
Hsien-Ming Wu Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Chang Gung University School of Medicine, Taoyuan, Taiwan

Search for other papers by Hsien-Ming Wu in
Google Scholar
PubMed
Close
,
Liang-Hsuan Chen Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Chang Gung University School of Medicine, Taoyuan, Taiwan

Search for other papers by Liang-Hsuan Chen in
Google Scholar
PubMed
Close
,
Wei-Jung Chiu Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Chang Gung University School of Medicine, Taoyuan, Taiwan

Search for other papers by Wei-Jung Chiu in
Google Scholar
PubMed
Close
, and
Chia-Lung Tsai Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital Linkou Medical Center, Chang Gung University School of Medicine, Taoyuan, Taiwan

Search for other papers by Chia-Lung Tsai in
Google Scholar
PubMed
Close

In this study, we investigate the effects of miRNA-138-5p and probable G-protein coupled receptor 124 (GPR124)-regulated inflammasome and downstream leukemia inhibitory factor (LIF)–STAT and adhesion molecule signaling in human decidual stromal cells. After informed consent was obtained from women aged 25–38 years undergoing surgical termination of the normal pregnancy and spontaneous miscarriage after 6–9 weeks of gestation, human decidual stromal cells were extracted from the decidual tissue. Extracellular vesicles (EVs) with microRNA (miRNA) between cells have been regarded as critical factors for embryo–maternal interactions on embryo implantation and programming of human pregnancy. MicroRNA-138-5p acts as the transcriptional regulator of GPR124 and the mediator of downstream inflammasome. LIF-regulated STAT activation and expression of integrins might influence embryo implantation. Hence, a better understanding of LIF–STAT and adhesion molecule signaling would elucidate the mechanism of microRNA-138-5p- and GPR124-regulated inflammasome activation on embryo implantation and pregnancy. Our results show that microRNA-138-5p, purified from the EVs of decidual stromal cells, inhibits the expression of GPR124 and the inflammasome, and activates the expression of LIF–STAT and adhesion molecules in human decidual stromal cells. Additionally, the knockdown of GPR124 and NLRP3 through siRNA increases the expression of LIF–STAT and adhesion molecules. The findings of this study help us gain a better understanding the role of EVs, microRNA-138-5p, GPR124, inflammasomes, LIF–STAT, and adhesion molecules in embryo implantation and programming of human pregnancy.

Open access
Mu-Hsin Chang Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC
Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Mu-Hsin Chang in
Google Scholar
PubMed
Close
,
Wei-Wen Kuo Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Wei-Wen Kuo in
Google Scholar
PubMed
Close
,
Ray-Jade Chen Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Ray-Jade Chen in
Google Scholar
PubMed
Close
,
Ming-Chin Lu Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Ming-Chin Lu in
Google Scholar
PubMed
Close
,
Fuu-Jen Tsai Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Fuu-Jen Tsai in
Google Scholar
PubMed
Close
,
Wu-Hsien Kuo Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Wu-Hsien Kuo in
Google Scholar
PubMed
Close
,
Ling-Yun Chen Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Ling-Yun Chen in
Google Scholar
PubMed
Close
,
Wen-Jun Wu Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Wen-Jun Wu in
Google Scholar
PubMed
Close
,
Chih-Yang Huang Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC
Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC
Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Chih-Yang Huang in
Google Scholar
PubMed
Close
, and
Chun-Hsien Chu Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC
Division of Cardiology, Institute of Medical and Molecular Toxicology, Department of Biological Science and Technology, Trauma and Emergency Center, Post-Baccalaureate School of Chinese Medicine, Department of Pediatrics, Division of Gastroenterology, Institute of Biochemistry and Biotechnology, Graduate Institute of Chinese Medical Science, Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, Armed Force Taichung General Hospital, Taichung, Taiwan, ROC

Search for other papers by Chun-Hsien Chu in
Google Scholar
PubMed
Close

The IGF-II/mannose 6-phosphate receptor (IGF2R) function in extracellular matrix (ECM) remodeling is known to occur as a result of transforming growth factor-β (TGF-β) activation and plasmin in the proteolytic cleavage level caused by the interaction between latent TGF-β and urokinase plasminogen activator receptor (uPAR) respectively. In one of our previous studies, we found IGF-II and IGF2R dose-dependently correlated with the progression of pathological hypertrophy remodeling following complete abdominal aorta ligation. However, how this IGF2R signaling pathway responds specifically to IGF-II and regulates the myocardial ECM remodeling process is unclear. We found that IGF2R was aberrantly expressed in myocardial infarction scars. The matrix metalloproteinase-9 (MMP-9) zymographic activity was elevated in H9c2 cardiomyoblast cells treated with IGF-II, but not IGF-I. Treatment with Leu27IGF-II, an IGF2R specifically binding IGF-II analog, resulted in significant time-dependent increases in the MMP-9, tissue-type plasminogen activator (tPA), and urokinase plasminogen activator (uPA); and a reduction in the tissue inhibitor of matrix metalloproteinases-2 (TIMP-2) protein expression. Furthermore, IGF2R expression inhibition by siRNA blocked the IGF-II-induced MMP-9 activity. We hypothesize that after IGF-II is bound with IGF2R, the resulting signal disrupts the balance in the MMP-9/TIMP-2 expression level and increases plasminogen activator (PAs) expression involved in the development of myocardial remodeling. If so, IGF2R signaling inhibition may have potential use in the development of therapies preventing heart fibrosis progression.

Free access
Ray-Jade Chen Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Ray-Jade Chen in
Google Scholar
PubMed
Close
,
His-Chin Wu Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by His-Chin Wu in
Google Scholar
PubMed
Close
,
Mu-Hsin Chang Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Mu-Hsin Chang in
Google Scholar
PubMed
Close
,
Chao-Hung Lai Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Chao-Hung Lai in
Google Scholar
PubMed
Close
,
Yun-Chen Tien Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Yun-Chen Tien in
Google Scholar
PubMed
Close
,
Jin-Ming Hwang Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC
Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Jin-Ming Hwang in
Google Scholar
PubMed
Close
,
Wu-Hsien Kuo Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Wu-Hsien Kuo in
Google Scholar
PubMed
Close
,
Fuu-Jen Tsai Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Fuu-Jen Tsai in
Google Scholar
PubMed
Close
,
Chang-Hai Tsai Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Chang-Hai Tsai in
Google Scholar
PubMed
Close
,
Li-Mien Chen Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Li-Mien Chen in
Google Scholar
PubMed
Close
,
Chih-Yang Huang Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC
Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC
Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Chih-Yang Huang in
Google Scholar
PubMed
Close
, and
Chun-Hsien Chu Trauma and Emergency Center, School of Medicine, Division of Cardiology, Graduate Institute of Chinese Medical Science, School of Applied Chemistry, Institute of Biochemistry and Biotechnology, Division of Gastroenterology, Department of Pediatrics, Department of Healthcare Administration, Division of Medical Technology, Graduate Institute of Basic Medical Science, Department of Health and Nutrition Biotechnology, China Medical University Hospital, Taichung 404, Taiwan, ROC

Search for other papers by Chun-Hsien Chu in
Google Scholar
PubMed
Close

This study examines the role of IGF2/mannose 6-phosphate receptor (IGF2R) signaling in the signaling transduction regulation and cell apoptosis in H9c2 cardiomyoblast cells. However, it is difficult to recognize the distinct activation of IGF2 signaling without interfacing with IGFI receptor (IGF1R) after exposure to IGF2. Leu27IGF2, an analog of IGF2 that interacts selectively with the IGF2R, was used to specifically activate IGF2R signaling in this study. DNA fragmentation and TUNEL assay revealed that in contrast to IGF1 treatment preventing angiotensin II and AG1024-induced cell apoptosis, Leu27IGF2 appears to synergistically increase apoptosis in those cells. We further found cell apoptosis induction and an increase in the active form of caspase 3 in the treatment of cells with Leu27IGF2, but not IGF1. To detect the interaction between IGF2R and Gαq using the immunoprecipitation assay, we found that IGF2R could directly interact with Gαq and after treatment with Leu27IGF2 the binding ability of Gαq to IGF2R had increased. This sequentially resulted in the phosphorylation of phospholipase C-β, a key downstream modulator of Gαq, on serine 537. Moreover, disruption of the Gαq protein by small interferon RNA reduced the cell apoptosis induced by Leu27IGF2. Our findings demonstrate that IGF2R activation appears to induce cell apoptosis via Gαq-deriving signaling cascades and its effect is completely different from IGF1R survival signaling.

Free access