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L C Murphy Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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B Peng Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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A Lewis Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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J R Davie Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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E Leygue Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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A Kemp Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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K Ung Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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M Vendetti Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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R Shiu Department of Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada
Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Mannitoba, Canada
Department of Physiology, University of Manitoba, Winnipeg, Manitoba, Canada

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To investigate the effect of altered oestrogen receptor (ER)α and ERβ expression on oestrogen and anti-oestrogen action in breast cancer, we have stably expressed an inducible ERβ1 in MCF7 breast cancer cells. Stably expressing clones were isolated and over-expression of ERβ1 correlated with increased levels of specific radiolabelled oestradiol (E2) binding. Increased ERβ1 did not affect endogenous levels of ERα but increased progesterone receptor (PR) levels. Over-expression of ERβ1 reduced growth responses to E2 in contrast to little if any effect of over-expression of ERα. In oestrogen-replete conditions, over-expression of ERβ1 but not ERα reduced proliferation. Over-expression of ERβ1 did not result in anti-oestrogen resistance but was associated with increased sensitivity to 4-hydroxytamoxifen. Our results suggested that over-expression of ERβ1 in the presence of an endogenously expressed ERα was associated with tamoxifen sensitivity but may negatively modulate ERα-mediated growth. However, not all ERα activities were inhibited since endogenous PR expression was increased by both ERα and ERβ1 over-expression. These data paralleled those seen in some in vivo studies showing a relationship between PR and ERβ expression as well as ERβ expression and tamoxifen sensitivity of ER-positive breast cancer patients. These models are relevant and will be useful for dissecting the role of ERβ1 expression in ER-positive breast cancer.

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