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Jan Wilde, Maria Erdmann, Michael Mertens, Gabriele Eiselt, and Martin Schmidt

. Cell fractionation and electrophoretic mobility shift assays To test for factors binding to the putative transcription factor-binding site, electrophoretic mobility shift assays (EMSAs) were carried out essentially as described previously ( Taylor et

Open access

Irina G Bogdarina, Peter J King, and Adrian J L Clark

phenylmethylsulfonylfluorid, 1 mM benzamidine, 30 mg/ml leupeptin, 5 mg/ml aprotinin, 5 mg/ml pepstatin). Nuclear extract was aliquoted and stored at −80 °C. Electrophoretic mobility shift assays (EMSAs) were performed in a 20 μl binding reaction containing 10 μg of the

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Kun Chen, Ji-Dan Zhou, Feng Zhang, Fang Zhang, Rui-Rui Zhang, Meng-Si Zhan, Xiao-Yin Tang, Bing Deng, Ming-Gang Lei, and Yuan-Zhu Xiong

-stranded oligonucleotides (Sangon, Shanghai, China) were designed and synthesized. The DNA binding activity of C/EBPβ protein was detected by LightShift Chemiluminescent EMSA Kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten microgram pig abdominal fat nuclear extract

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Krishan Johansson-Haque, Elanchelian Palanichamy, and Sam Okret

transfection experiments to test for functional activity. Electrophoretic mobility shift assay (EMSA) was also used to study protein–DNA interactions at the GC-responsive region of the hDUSP1 promoter region. Materials and methods Cells and growth conditions

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Nobuko Kimura, Nobuko Takamatsu, Yoshio Yaoita, R Yoshiyuki Osamura, and Narimichi Kimura

assayed. The promoterless pGL3-Basic vector was included as a control in the transfection experiments and the results of the luciferase activity were calculated relative to the activity of pGL3-Basic. Electrophoretic mobility shift assay (EMSA) EMSA was

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Kristy A Brown, Khampoune Sayasith, Nadine Bouchard, Jacques G Lussier, and Jean Sirois

system using the ImageQuant software version 1.1 (Molecular Dynamics, Amersham Biosciences). Granulosa cell nuclear extracts and electrophoretic mobility shift assays (EMSAs) Equine granulosa cells were obtained from

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Katarzyna Zielniok, Agnieszka Sobolewska, and Małgorzata Gajewska


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Feng Zhang, Qi Xiong, Hu Tao, Yang Liu, Nian Zhang, Xiao-Feng Li, Xiao-Jun Suo, Qian-Ping Yang, and Ming-Xin Chen

luciferase reporter assays could follow a previously described method ( Zhang et al. 2018 ) . Electrophoretic mobility shift assays For electrophoretic mobility shift assays (EMSAs), nuclear proteins (NPs) were extracted from bovine longissimus

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D Bouton, H Escriva, R L de Mendonça, C Glineur, B Bertin, C Noël, M Robinson-Rechavi, A de Groot, J Cornette, V Laudet, and R J Pierce

(EMSA) BgRXR cDNA was cloned into XhoI/KpnI digested pTL1 (a modified version of pSG5; Stratagene, La Jolla, CA, USA), for transient transfection assays and in vitro translation. Vectors expressing potential heterodimer partners were as

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Xueting Wang, Zhiran Zou, Zhihui Yang, Shan Jiang, Yapeng Lu, Dan Wang, Zhangji Dong, Sha Xu, and Li Zhu

. Products were analyzed by 1.5% agarose gel electrophoresis and quantified by real-time PCR. Fold enrichment = 2 Ct (IgG)−Ct (HIF1α) . Electrophoretic mobility shift assay (EMSA) and supershift assays EMSA was carried out to determine HIF1