Search Results

You are looking at 1 - 1 of 1 items for

  • Author: Heidi Jamin x
  • Refine by access: Content accessible to me x
Clear All Modify Search
Isabelle Durrer Department of Nephrology and Hypertension University of Bern, Berne, Switzerland

Search for other papers by Isabelle Durrer in
Google Scholar
PubMed
Close
,
Daniel Ackermann Department of Nephrology and Hypertension University of Bern, Berne, Switzerland

Search for other papers by Daniel Ackermann in
Google Scholar
PubMed
Close
,
Rahel Klossner Department of Nephrology and Hypertension University of Bern, Berne, Switzerland
Department of Internal Medicine, Sonnenhof, Lindenhofgruppe, Berne, Switzerland

Search for other papers by Rahel Klossner in
Google Scholar
PubMed
Close
,
Michael Grössl Department of Nephrology and Hypertension University of Bern, Berne, Switzerland

Search for other papers by Michael Grössl in
Google Scholar
PubMed
Close
,
Clarissa Vögel Department of Nephrology and Hypertension University of Bern, Berne, Switzerland

Search for other papers by Clarissa Vögel in
Google Scholar
PubMed
Close
,
Therina Du Toit Department for BioMedical Research University of Bern, Berne, Switzerland

Search for other papers by Therina Du Toit in
Google Scholar
PubMed
Close
,
Bruno Vogt Department of Nephrology and Hypertension University of Bern, Berne, Switzerland

Search for other papers by Bruno Vogt in
Google Scholar
PubMed
Close
,
Heidi Jamin Department of Nephrology and Hypertension University of Bern, Berne, Switzerland
Department for BioMedical Research University of Bern, Berne, Switzerland

Search for other papers by Heidi Jamin in
Google Scholar
PubMed
Close
,
Markus G Mohaupt Department of Internal Medicine, Sonnenhof, Lindenhofgruppe, Berne, Switzerland
Department for BioMedical Research University of Bern, Berne, Switzerland

Search for other papers by Markus G Mohaupt in
Google Scholar
PubMed
Close
, and
Carine Gennari-Moser Department of Nephrology and Hypertension University of Bern, Berne, Switzerland
Department for BioMedical Research University of Bern, Berne, Switzerland

Search for other papers by Carine Gennari-Moser in
Google Scholar
PubMed
Close

Extra-adrenal de novo aldosterone (Aldo) production has been described inconsistently. Systematic data based upon state-of-the-art technology including validated controls are sparse. We hypothesized that aldosterone synthase (CYP11B2) expression and de novo Aldo production are absent in nonadrenal human cell lines, either immortalized cell lines or commercially available primary cell lines, including peripheral blood mononuclear cells (PBMCs) of individuals without and with primary hyperaldosteronism (PA). CYP11B2-transfected COS-7 and endogenous CYP11B2 expressing adrenal H295R cells served as positive controls. Various well-characterized, purchased, immortalized (BeWo, HEK293, HTR-8/SVneo, JEG-3) and primary (HAEC, HLEC, HRGEC, HRMC, HUAEC, HUVEC, PBMC) cell lines as well as self-isolated PBMCs from PA patients (n = 5) were incubated with the steroid hormone substrates progesterone, deoxycorticosterone, corticosterone or 18-OH-corticosterone with and without Ang II for 24 h to assess CYP11B2 enzymatic activity. CYP11B2 expression was analyzed by real-time PCR and liquid chromatography–mass spectrometry was used to quantify Aldo production. Pronounced CYP11B2 mRNA expression and Aldo production were observed in both positive controls, which followed an incremental time course. Neither substrates alone nor coincubation with Ang II significantly stimulated CYP11B2 expression or Aldo production in various immortalized and primary cell lines and PBMCs of PA patients. These results strongly support the absence of relevant de novo extra-adrenal Aldo production in nonadrenal cells, including blood mononuclear cells, irrespective of the absence or presence of autonomous adrenal Aldo production.

Open access