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Irina G Bogdarina, Peter J King, and Adrian J L Clark

phenylmethylsulfonylfluorid, 1 mM benzamidine, 30 mg/ml leupeptin, 5 mg/ml aprotinin, 5 mg/ml pepstatin). Nuclear extract was aliquoted and stored at −80 °C. Electrophoretic mobility shift assays (EMSAs) were performed in a 20 μl binding reaction containing 10 μg of the

Open access

Feng Zhang, Qi Xiong, Hu Tao, Yang Liu, Nian Zhang, Xiao-Feng Li, Xiao-Jun Suo, Qian-Ping Yang, and Ming-Xin Chen

luciferase reporter assays could follow a previously described method ( Zhang et al. 2018 ) . Electrophoretic mobility shift assays For electrophoretic mobility shift assays (EMSAs), nuclear proteins (NPs) were extracted from bovine longissimus

Open access

Silvia Ottaviani, Greg N Brooke, Ciara O'Hanlon-Brown, Jonathan Waxman, Simak Ali, and Laki Buluwela

placed in boiling water, which was allowed to cool at room temperature overnight. For electrophoretic mobility shift assay (EMSA), 2.5 μl COS-1 HSB extract were pre-incubated with 142 ng/μl poly(deoxyinosine-deoxycytosine) (dI.dC) for 30 min at 4 °C. For

Open access

Tien-Chun Yang, Mei-Hua Lu, Wei-Jie Wang, and Jang-Yi Chen

( Fig. 3A ). Electrophoretic mobility shift assay (EMSA) results showed that the enhanced region could bind to nuclear extract proteins of SHR VSMCs ( Fig. 3B , lane 2) but not to WKY VSMCs ( Fig. 3B , lane 4). These results suggest that SHR VSMCs are