Search Results

You are looking at 31 - 40 of 179 items for :

  • "ubiquitin" x
  • Refine by Access: All content x
Clear All
Free access

Christopher C Valley, Natalia M Solodin, Ginny L Powers, Stephanie J Ellison, and Elaine T Alarid

has been established that estrogen-induced ERα degradation is mediated by the ubiquitin–proteasome pathway, and this process is effectively blocked by pharmacologic inhibitors of the 26S proteasome, such as ALLnL, lactacystin, and MG132 ( Alarid et al

Free access

Matthew L Rise, Susan E Douglas, Dionne Sakhrani, Jason Williams, K Vanya Ewart, Marlies Rise, William S Davidson, Ben F Koop, and Robert H Devlin

. Transcripts of interest and a normalizer gene were selected from the results of microarray experiments. For both the 3.5K and 16K GRASP studies, five different microarray features with ESTs identified as ubiquitin (top BLASTX hit: Oncorhynchus mykiss

Free access

Hayden Weng Siong Tan, Arthur Yi Loong Sim, Su Ling Huang, Ying Leng, and Yun Chau Long

muscle-specific E3 ubiquitin ligases, MuRF1 and atrogin-1 ( Fig. 5 D), which are involved in proteasomal proteolysis. We next tested whether this effect could be sustained under serum-starved conditions, as this is routinely used to induce atrophy and

Free access

Valérie Baldacchino, Sylvie Oble, Patrick-Olivier Décarie, Isabelle Bourdeau, Pavel Hamet, Johanne Tremblay, and André Lacroix

of adult and fetal tissues was purchased from Clontech (BD Bioscience, Palo Alto, CA, USA). An hGIP-R cDNA probe corresponding to nucleotides 387–1195 and a ubiquitin probe (Clontech) were labeled with [α- 32 P]dCTP, using the Klenow fragment

Free access

Arnaud Droit, Guy G Poirier, and Joanna M Hunter

interactions, each has its limitations ( Wehrman et al. 2002 ). Clearly, the β-lactamase approach has great potential for determining protein interactions in mammalian cells. Another approach called ubiquitin-based split-protein sensor (USPS) makes

Free access

S Bauersachs, S E Ulbrich, K Gross, S E M Schmidt, H H D Meyer, R Einspanier, H Wenigerkind, M Vermehren, H Blum, F Sinowatz, and E Wolf

; reverse 5′-TTTTCTTTTTCTTGCCGGG (148 bp)), SOX4 (forward 5′-ATTTCGAGTTCCCGGACTAC; reverse 5′-CCTTCAGTAGGTGAAGACCAGG (101 bp)), UTMP (forward 5′-ATATCATCTTCTCCCCCATGG; reverse 5′-GTGCACATCCAACAGTTTGG (126 bp)), and the housekeeping gene Ubiquitin

Free access

M J Waters, H N Hoang, D P Fairlie, R A Pelekanos, and R J Brown

endoplasmic reticulum and is required for ubiquitin system-dependent endocytosis. PNAS 99 9858 –9863. Harding PA , Wang X, Okada S, Chen WY, Wan W & Kopchick JJ 1996 Growth hormone (GH) and a GH antagonist promote GH receptor

Open access

Irit Hochberg, Innocence Harvey, Quynh T Tran, Erin J Stephenson, Ariel L Barkan, Alan R Saltiel, William F Chandler, and Dave Bridges

al . 2007 ). Exposure of rats to glucocorticoids activates the muscle ubiquitin-proteasome system ( Wing & Goldberg 1993 , Price et al . 1994 ), increasing muscle expression of proteases (cathepsins B and D, calpain) and components of the ubiquitin

Free access

Denis Nonclercq, Fabrice Journé, Ioanna Laïos, Carole Chaboteaux, Robert-Alain Toillon, Guy Leclercq, and Guy Laurent

. 1999 , 2006 ; Lonard et al. 2000 ; Journé et al. 2004 ; Laïos et al. 2005 ), ER breakdown involves the ubiquitin–proteasome pathway. Modalities of ER degradation can, however, vary according to the receptor conformation, which is itself

Free access

Lichun Zhou, Baohua Ma, and Xiuzhen Han

). Expansion of autophagy The phagophore elongation and formation of complete autophagosomes require two ubiquitin-like protein conjugation systems: ATG12-ATG5-ATG16L1 and microtubule-associated light chain-3 (LC3)-phosphatidylethanolamine (PE) ( Jia & Sowers