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) Hsc70 cochaperone complexes that promote client protein ubiquitination and proteasomal degradation are illustrated. CHIP interacts with Hsc70 via a TPR domain and acts as an ubiquitin ligase for Hsc70 clients, recruiting ubiquitin conjugating enzyme

regulated by miR-23a-3p, we further investigated the effect of miR-23a-3p on the ubiquitination of β-catenin. The IP assay indicated the binding between β-catenin and CUL3 ( Fig. 4A ). After overexpressing miR-23a-3p in Ishikawa cells, the ubiquitin

( Table 1 ). The four missense mutations (p.Cys340Gly, p.Arg365Ser, p.Phe417Ile, and p.His420Gln) were located within a zinc finger motif or a RING finger motif ( Fig. 1 ), regions predicted to be involved in RNA binding and ubiquitin ligase activity of

Introduction TNFAIP3 (tumour necrosis factor alpha-induced protein 3) encodes a zinc finger protein called A20 that exerts dual ubiquitin editing properties ( Wertz et al. 2004 ). A20 is an endogenous negative regulator of inflammatory and

transcription elongation factor B (SIII) polypeptide 2, plectin 1, and ubiquitin-conjugating enzyme E2G 2, showed the same gender difference. The only gene showing the opposite result between these two studies was Fabp4. Thus, we performed QRT-PCR analysis on

–Fbox (SCF) complex that is responsible for ubiquitin-mediated proteasomal degradation ( Dias et al . 2002 ). The ubiquitin-mediated proteasomal degradation pathway is required for GH receptor endocytosis and regulates downstream signalling molecules

transcript stability and translation might contribute to the high AT1R synthesis observed in hyperthyroidism. An additional mechanism that tightly controls the level of many key proteins involved in transcriptional regulation is the ubiquitin

Santa Cruz Biotechnology. Mouse monoclonal antibody against ubiquitin was from Covance Research Products Inc (Denver, PA, USA). Monoclonal anti-ubiquitin antibody was provided from Medical & Biological Laboratories Co. (Nagoya, Japan). Rabbit anti-ubiquitin

transferases, methyl transferases as well as ubiquitin ligases, all of which contribute to the dynamic functions of the coactivators ( Malovannaya et al . 2010 ). Recent studies on coregulator dynamics have identified some novel mechanisms for ER

( Huber et al . 2005 , Hanson et al . 2009 , 2011 b , 2012 ). CUL7 forms the central component of an SCF E3 ubiquitin ligase ( Dias et al . 2002 ) that localises to the Golgi apparatus ( Litterman et al . 2011 ) and has been shown to be involved in