receptors ( Chen et al . 1997 ). In all of these studies, the inhibition of nuclear receptor transactivation activity is associated with mechanisms involving heterodimerization formation that have been demonstrated through gel shift (EMSA) experiments ( Liu
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Pia Kiilerich, Gérard Triqueneaux, Nynne Meyn Christensen, Vincent Trayer, Xavier Terrien, Marc Lombès, and Patrick Prunet
S Zhang, X Li, R Burghardt, R Smith III, and S H Safe
electrophoretic mobility shift assay (EMSA) The probe containing a consensus estrogen-responsive element (ERE; 5′-GTCCAAAGTCAGGTCACAGTGACCTGATCAAAGTT-3′) was synthesized, annealed and 32 P-labeled at the 5′-end using T4 polynucleotide kinase (Roche) and
Young Sun Kang, Yun Gyu Park, Bo Kyung Kim, Sang Youb Han, Yi Hwa Jee, Kum Hyun Han, Mi Hwa Lee, Hye Kyoung Song, Dae Ryong Cha, Shin Wook Kang, and Dae Suk Han
μl color reagent per well. The intensity of the color was measured in an ELISA reader at 540 nm. Electrophoretic mobility shift assay (EMSA) Podocytes were treated with 100 nM angiotensin II for 8 h. Nuclear
Luc J Martin and Jacques J Tremblay
& Tremblay 2008 , Martin et al . 2008 ), in vitro approaches such as EMSA showed a weak binding of NUR77 to the −95 bp SF1/NBRE element ( Martin et al . 2008 ), suggesting that recruitment of NUR77 to the Star promoter might be mediated by interactions
Suryaprakash Raichur, Patrick Lau, Bart Staels, and George E O Muscat
cell line. Whether RORγ regulates the LPL promoter by primary (direct) and/or secondary mechanisms remain unclear at present. Future studies utilizing ChIP, deletion and EMSA analysis will be used to address these questions. These latter
Zhen-Yu She and Wan-Xi Yang
assay (EMSA) and ChIP demonstrate that SOX9 recognizes and binds to the paired SOX-binding sites within the Pgd 2 promoter and then regulates the expression of Pgd 2 to ensure the sufficient Sertoli cells differentiation during normal sex development
