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See-Tong Pang, Wen-Chi Hsieh, Cheng-Keng Chuang, Chun-Hsiang Chao, Wen-Hui Weng, and Horng-Heng Juang

electrophoretic mobility-shift assay (EMSA) was performed as described previously ( Tsui et al . 2006 ). The double-stranded DNA fragment containing the putative ChoRE (5′-TGTGCACGAGGGCAGCACGAGCCTC-3′), was 5′-end labeled with γ-P 32 ATP using T4 polynucleotide

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Edward T Bagu and Manuela M Santos


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Jens Vanselow, Wei Yang, Jens Herrmann, Holm Zerbe, Hans-Joachim Schuberth, Wolfram Petzl, Wolfgang Tomek, and Hans-Martin Seyfert

). Electrophoretic mobility shift assay (EMSA) Preparation of nuclear extracts, probe labelling, super shifts and the general procedures for the EMSA analysis were conducted as described ( Mao et al. 2002 ). To generate the double-stranded probe, we used

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Xianjie Wen and Yisheng Yang

, suggesting a cross-talk between the GLIS3 and GLI signaling pathways ( Kim et al . 2003 ). To identify the specific binding site for GLIS3, Beak and coworkers combined PCR and electrophoretic mobility shift assay (EMSA) to screen a mixture of 60 bp

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Marie-Hélène Quentien, Véronique Vieira, Maurice Menasche, Jean-Louis Dufier, Jean-Paul Herman, Alain Enjalbert, Marc Abitbol, and Thierry Brue

Electrophoretic mobility shift assays (EMSA) were carried out with wild-type and mutant PITX2 and the 32 P-labeled double-stranded oligonucleotide 5′-ACCAGGATGC TAAGCC TGTGTC-3′, containing the CE3 PITX-specific binding site (in bold) of the proopiomelanocortin

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Ann Lo, Weiming Zheng, Yimei Gong, John R Crochet, and Lisa M Halvorson

and 7), while the mutated oligonucleotide had limited effect (lanes 4 and 8). Figure 6 Both endogenous and in vitro translated GATA proteins bind to position −101 of the rat LHβ gene promoter. (A) Electrophoretic mobility shift assay (EMSA

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Carlos Gaspar, Jonás I Silva-Marrero, María C Salgado, Isabel V Baanante, and Isidoro Metón

E-box at positions −10 to −5 relative to the transcriptional start. Bearing in mind that Usf2 transactivates numerous genes by binding to E-boxes ( Corre & Galibert 2005 ), electrophoretic mobility shift assays (EMSA) were performed with nuclear

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Marie France Bouchard, Hiroaki Taniguchi, and Robert S Viger

). Electrophoretic mobility shift assay Recombinant GATA4 wild-type and mutated proteins were in vitro translated using a QuickCoupled TNT in vitro transcription and translation system (Promega). Electrophoretic mobility shift assays (EMSA; DNA-binding) were

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Russell Snyder and Thomas Thekkumkara

's improved minimal essential medium was obtained from Cellgro-Mediatech, Inc. (Manassas, VA, USA). Fetal bovine serum (FBS) was from Equitech-Bio, Inc. (Kerrville, TX, USA). Oligonucleotide primers and EMSA nucleotide duplex probes were obtained from

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Man Huang, Duraisamy Kempuraj, Nikoletta Papadopoulou, Taxiarchis Kourelis, Jill Donelan, Akrivi Manola, and Theoharis C Theoharides

EMSA and effects of α-helical (9–41) CRH, SB203580 (10 μM) and PD98059 (20 μM). (B) Supershift assay using antibody against the p50 subunit of NF-κB confirmed that the Ucn-stimulated increase in binding resulted from NF-κB activity in the nuclear