Nucleotide sequence of the cDNA encoding the common α subunit of the chicken pituitary glycoprotein hormones

in Journal of Molecular Endocrinology
Authors:
D. N. Foster
Search for other papers by D. N. Foster in
Current site
Google Scholar
PubMed
Close
,
D. Galehouse
Search for other papers by D. Galehouse in
Current site
Google Scholar
PubMed
Close
,
T. Giordano
Search for other papers by T. Giordano in
Current site
Google Scholar
PubMed
Close
,
B. Min
Search for other papers by B. Min in
Current site
Google Scholar
PubMed
Close
,
I. C. Lamb
Search for other papers by I. C. Lamb in
Current site
Google Scholar
PubMed
Close
,
D. A. Porter
Search for other papers by D. A. Porter in
Current site
Google Scholar
PubMed
Close
,
K. J. Intehar
Search for other papers by K. J. Intehar in
Current site
Google Scholar
PubMed
Close
, and
W. L. Bacon
Search for other papers by W. L. Bacon in
Current site
Google Scholar
PubMed
Close
View More View Less
Restricted access

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $1.00
USD  $1.00

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $0.01
USD  $0.01

USD  $1.00
USD  $1.00

USD  $1.00
USD  $1.00

USD  $1.00
USD  $1.00

USD  $1.00
USD  $1.00

USD  $1.00
USD  $1.00

ABSTRACT

Recombinant cDNA clones that encode the α subunit of the chicken pituitary glycoprotein hormones were isolated from a pituitary library. The longer of the two cDNA clones that were sequenced was 754bp in length. It contained 81 nucleotides of the 5′-untranslated region (UTR), an open-reading frame of 360bp that encoded a 24 amino acid leader polypeptide sequence as well as the 96 amino acid mature α subunit, and 268 nucleotides of the 3′-UTR, followed by a 45 bp poly(A) tract. There was 69–79% homology between the nucleotide sequence of the coding region for the chicken and mammalian α-subunit cDNAs. Northern blot analysis revealed that the steady-state levels of an approximately 800 bp α-subunit specific transcript increased quantitatively when dispersed chicken pituitary glands were treated in culture with chicken gonadotrophin-releasing hormone-I.