circ_0134120: a new frontier in understanding postmenopausal osteoporosis pathogenesis

in Journal of Molecular Endocrinology
Authors:
Junling Wang Gynecologic Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China

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https://orcid.org/0009-0008-6847-8525
,
Hongyan Zhang Gynecologic Department, Hainan Traditional Chinese Medicine Hospital, Haikou, China

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Yue Cao Science and technology department, Guangzhou University of Chinese Medicine, Guangzhou, China

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Irene Ma Gynecologic Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China

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Xuefang Liang Gynecologic Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China

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, and
Dongfang Xiang Gynecologic Department, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China

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Correspondence should be addressed to D Xiang or X Liang: Dongfangx@126.com or liangxuefang@gzucm.edu.cn

*(J Wang and H Zhang contributed equally to this work)

DOI:
https://doi.org/10.1530/JME-23-0140
Volume/Issue:
Volume 73: Issue 1
Article Type:
Research Article
Article ID:
e230140
Online Publication Date:
29 Apr 2024
Copyright:
© the author(s) 2024
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Postmenopausal osteoporosis (OP) is a prevalent skeletal disease with not fully understood molecular mechanisms. This study aims to investigate the role of circular RNA (circRNA) in postmenopausal OP and to elucidate the potential mechanisms of the circRNA–miRNA–mRNA regulatory network. We obtained circRNA and miRNA expression profiles from postmenopausal OP patients from the Gene Expression Omnibus database. By identifying differentially expressed circRNAs and miRNAs, we constructed a circRNA–miRNA–mRNA network and identified key genes associated with OP. Further, through a range of experimental approaches, including dual-luciferase reporter assays, RNA pull-down experiments, and qRT-PCR, we examined the roles of circ_0134120, miR-590-5p, and STAT3 in the progression of OP. Our findings reveal that the interaction between circ_0134120 and miR-590-5p in regulating STAT3 gene expression is a key mechanism in OP, suggesting the circRNA–miRNA–mRNA network is a potential therapeutic target for this condition.

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Online ISSN:
1479-6813
Print ISSN:
0952-5041
Copyright:
© the author(s) 2024
Article ID:
e230140
Article Type:
Research Article
Received Date:
20 Nov 2023
Accepted Date:
02 Apr 2024
Print Publication Date:
01 Jul 2024
Online Publication Date:
29 Apr 2024
Keywords:
circRNA; JAK-STAT signaling pathway; miRNA; mRNA; postmenopausal osteoporosis; STAT3

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