Son of sevenless 1 (SOS1), the RasGEF, interacts with ERα and STAT3 during embryo implantation

in Journal of Molecular Endocrinology
Authors:
Renjini A PadmanabhanFemale Reproduction and Metabolic Syndromes Laboratory, Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India

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Damodaranpillai P ZyjuFemale Reproduction and Metabolic Syndromes Laboratory, Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India

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Anand G SubramaniamFemale Reproduction and Metabolic Syndromes Laboratory, Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India

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Jaya NautiyalInstitute of Reproductive and Developmental Biology, Department of Surgery and Cancer, Imperial College, London, UK

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Malini LalorayaFemale Reproduction and Metabolic Syndromes Laboratory, Division of Molecular Reproduction, Rajiv Gandhi Centre for Biotechnology, Poojappura, Thiruvananthapuram, Kerala, India

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https://orcid.org/0000-0003-4985-8932
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Correspondence should be addressed to M Laloraya: laloraya@rgcb.res.in

*(R A Padmanabhan and D P Zyju contributed equally to this work)

DOI:
https://doi.org/10.1530/JME-22-0089
Volume/Issue:
Volume 70: Issue 1
Article Type:
Research Article
Article ID:
e220089
Online Publication Date:
07 Dec 2022
Copyright:
© Society for Endocrinology 2022
Restricted access

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Estrogen accounts for several biological processes in the body; embryo implantation and pregnancy being one of the vital events. This manuscript aims to unearth the nuclear role of Son of sevenless1 (SOS1), its interaction with estrogen receptor alpha (ERα), and signal transducer and activator of transcription 3 (STAT3) in the uterine nucleus during embryo implantation. SOS1, a critical cytoplasmic linker between receptor tyrosine kinase and rat sarcoma virus signaling, translocates into the nucleus via its bipartite nuclear localization signal (NLS) during the ‘window of implantation’ in pregnant mice. SOS1 associates with chromatin, interacts with histones, and shows intrinsic histone acetyltransferase (HAT) activity specifically acetylating lysine 16 (K16) residue of histone H4. SOS1 is a coactivator of STAT3 and a co-repressor of ERα. SOS1 creates a partial mesenchymal–epithelial transition by acting as a transcriptional modulator. Finally, our phylogenetic tree reveals that the two bipartite NLS surface in reptiles and the second acetyl coenzymeA (CoA) (RDNGPG) important for HAT activity emerges in mammals. Thus, SOS1 has evolved into a moonlighting protein, the special class of multi-tasking proteins, by virtue of its newly identified nuclear functions in addition to its previously known cytoplasmic function.

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Print ISSN:
0952-5041
Online ISSN:
1479-6813
Copyright:
© Society for Endocrinology 2022
Article ID:
e220089
Article Type:
Research Article
Received Date:
09 Sep 2022
Accepted Date:
14 Sep 2022
Print Publication Date:
01 Jan 2023
Online Publication Date:
07 Dec 2022
Keywords:
embryo implantation; estrogen receptors; SOS1; histone acetyltransferase; moonlighting proteins

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