Downregulation of miR-146b-5p via iodine involvement repressed papillary thyroid carcinoma cell proliferation

in Journal of Molecular Endocrinology
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  • 1 Key Lab of Etiology and Epidemiology, National Health and Family Planning Commission, Center for Endemic Disease Control, Chinese Center for Disease Control and Prevention, Harbin Medical University, Harbin, Heilongjiang, China
  • 2 Department of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, Heilongjiang, China
  • 3 College of Medical Laboratory Science, Guilin Medical University, Guilin, Guangxi, China
  • 4 Department of Radiation Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China
  • 5 Department of Thyroid Surgery, First Affiliated Hospital of Henan University, Kaifeng, Henan, China
  • 6 Kaifeng Center for Disease Prevention and Control, Kaifeng, Henan, China
  • 7 Department of Gastrointestinal Medical Oncology, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China

Correspondence should be addressed to Y Liu or X Yu: ly-da@163.com or yxg301@163.com

*(Y Pan and W Yun contributed equally to this work)

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miR-146b-5p is overexpressed in papillary thyroid carcinoma (PTC) and is thought to be a related diagnostic marker. Previous studies have indicated the effects of iodine on oncogenic activation. However, the effect of iodine on the proliferation of PTC cells and the associated underlying mechanisms remain unclear. We found that miR-146b-5p was downregulated and smad4 was upregulated in patients exposed to high iodine concentration by in situ hybridisation (ISH) and immunohistochemical (IHC). NaI (10−3 M) treatment downregulated miR-146b-5p and upregulated Smad4 in PTC cell lines. Luciferase assay was used to confirm that Smad4 is a target of miR-146b-5p. Furthermore, MTT assay and cell cycle analysis indicated that 10−3 M NaI suppressed cell proliferation and caused G0/G1 phase arrest. Real-time PCR and Western blotting demonstrated that 10−3 M NaI increased p21, p27, and p57 levels and reduced cyclin D1 levels in PTC cells. Our findings suggest that 10−3 M NaI increases Smad4 levels through repression of miR-146b-5p expression, curbing the proliferation in PTC.

Supplementary Materials

    • Downregulation of miR-146b-5p via iodine involvement repressed papillary thyroid carcinoma cell proliferation
    • Supplementary Table 1. Associations between iodine levels and of miR-146b-5p expression papillary thyroid carcinoma patients
    • Supplementary Figure 1 The expression of NIS is higher in high iodine areas than in adequate areas. (A) H&E showing the features of thyroid follicular cells and papillary thyroid carcinoma (20&#x00D7;); (B) RT-PCR was performed to determine the expression of miR-146b-5p in tissues. Relative levels were normalised to those of U6. # p < 0.05 vs Adequate iodine. (C) Expression of NIS in adequate iodine and high iodine areas (Immunohistochemistry, 20&#x00D7;).
    • Supplementary Figure 2 10-3 M NaI promotes Smad4 expression via miR-146b-5p repression in PTC cells. Luciferase assay showed that miR-146b-5p could target Smad4 with or without 10-3 M NaI treatment. n = 3, # p < 0.05.
    • Supplementary Figure 3 10-3 M NaI reduces viability and proliferation in PTC cells. (A) MTT assay, (B) Cell counting tests determined cell proliferation and viability at 24, 48, 72, and 96 h. n = 3. Data are presented as the mean &#x00B1; SD of three independent experiments. * p < 0.0167 vs. con.
    • Supplementary Figure 4 10-3 M NaI promotes p21 and p57 expression in PTC cells. Western blot analysis for p21 and p57 in cells treated with 10-3 M NaI. Data are presented as means &#x00B1; SD of three independent experiments. # p < 0.05 vs con.
    • Supplementary Figure 5 10-3 M NaI promotes p21, p27, p57 and cyclin D1 expression in PTC tissues. RT-PCR was performed to determine the expression of p21, p27, p57 and cyclin D1. GAPDH was used as an internal control. Data are presented as means &#x00B1; SD of three independent experiments. # p < 0.05 vs Adequate iodine.
    • Supplementary Figure 6 10-3 M NaI upregulates thyroid-specific genes in PTC cells. (A and C) The mRNA and protein expression of TG in TPC-1 cells. No detectable levels of NIS, TSHR and TPO were observed in TPC-1 cells. (B and D) The mRNA and protein expression of TG and TSHR in BCPAP cells. No detectable levels of NIS and TPO were observed in BCPAP cells. Data are presented as means &#x00B1; SD of three independent experiments. # p < 0.05 vs Control.
    • Supplementary Figure 7 10-3 M NaI inhibits proliferation in PTC cells via miR-146b-5p. MTT assays determined cell proliferation at 24, 48, and 72 h. n = 3. Data are presented as the mean &#x00B1; SD of three independent experiments. # p < 0.05 vs con, ## p < 0.05 vs NaI.

 

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