HOXA10 co-factor MEIS1 is required for the decidualization in human endometrial stromal cell

in Journal of Molecular Endocrinology
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Yawen Xu Department of Nutrition and Food Hygiene, School of Health Science, Wuhan University, Wuhan, Hubei, China

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Jinhua Lu Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China
Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China

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Jinxiang Wu Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China

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Ruiwei Jiang Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China

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Chuanhui Guo Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China

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Yedong Tang Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China

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Haibin Wang Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China
Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China

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Shuangbo Kong Reproductive Medical Center, The First Affiliated Hospital of Xiamen University, Xiamen, Fujian, China
Fujian Provincial Key Laboratory of Reproductive Health Research, School of Medicine, Xiamen University, Xiamen, Fujian, China

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Suqing Wang Department of Nutrition and Food Hygiene, School of Health Science, Wuhan University, Wuhan, Hubei, China
Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Academy for Preventive Medicine, Wuhan, Hubei, China

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Correspondence should be addressed to S Kong or S Wang: shuangbo_kong@163.com or swang2099@whu.edu.cn
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Decidualization is a critical process for embryo implantation and pregnancy maintenance in humans. The homeobox gene HOXA10 has been widely studied in endometrial receptivity establishment and decidualization. MEIS1, a three-amino-acid loop extension (TALE) family homeobox gene, has been proven to be a co-factor for HOXA10 in mouse uterus. However, the interaction between MEIS1 and HOXA10 in the human decidual cells remains to be elucidated. siRNA and CRISPR-Cas9 were employed to knockdown and knockout MEIS1 in the cultured human endometrial stromal cells, and it was found that MEIS1 deficiency leads to impaired decidualization. The physical interaction between the MEIS1 and HOXA10 in human endometrial stromal cell was confirmed by immunoprecipitation. Moreover, KAT2B and ETA were proved to be downregulated in the absence of MEIS1, and luciferase reporter and ChIP assays demonstrated that MEIS1-HOXA10 complex binds to the promoters of KAT2B and ETA and regulates their activity. Overexpression of KAT2B and ETA can partially rescue the decidualization defects in MEIS1-knockout HESCs. Taken together, these data suggest that MEIS1 plays an indispensable role in decidualization in human endometrial stromal cells, and MEIS1 interacts with HOXA10 to regulate the downstream genes, such as KAT2B and ETA. These findings will contribute to our understanding about the regulatory network in the process of decidualization in humans.

Supplementary Materials

    • Supplementary Figure 1. The expressions of ET-1 are not different between with and without MEIS1. Relative expressions of ET-1 in WT and KO in hESCs treated with differentiation medium were assayed by real-time PCR. n=3.

 

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