FNDC5/Irisin counteracts lipotoxic-induced apoptosis in hypoxic H9c2 cells

in Journal of Molecular Endocrinology

Correspondence should be addressed to I Moscoso or R Lage: imosgal@gmail.com or rlagef@gmail.com

*(I Moscoso and M Cebro-Márquez contributed equally to this work)

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Irisin is a newly identified adipokine critical to modulate body metabolism, fatty acid metabolism and oxidative stress; recent evidence suggests a cardioprotective role in ischemic injury. Loss of cardiomyocytes during acute myocardial infarction is strongly associated with energetic changes and lipotoxic-induced apoptosis. Our aim was to study FNDC5/irisin’s potential protective role against hypoxia and lipotoxicity, both related with myocardial infarction environment. H9c2 cells were treated with palmitate and/or irisin in normoxic/hypoxic conditions. Cell viability and apoptosis were assessed by MTT assay and annexin V/PI staining. Immunoblotting was used to confirm apoptotic cascade regulation. Irisin counteracts lipotoxic-induced apoptosis in hypoxic cardiomyoblasts by activating Akt signaling pathway suggesting the potential therapeutic role of irisin in ischemic heart disease.

 

      Society for Endocrinology

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