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Semi-quantitative analysis of interleukin-1α, interleukin-6 and interleukin-8 mRNA expression by human thyrocytes
in Journal of Molecular EndocrinologyABSTRACT
It has been suggested that the thyroid itself may contribute to the inflammatory process observed in autoimmune thyroiditis by releasing the cytokines interleukin-1α (IL-1α), interleukin-6 (IL-6) and interleukin-8 (IL-8), but studies of cytokine gene expression in thyrocytes have been limited and conflicting. A semi-quantitative reverse transcription-PCR technique has been used to investigate the expression of IL-1α, IL-6 and IL-8 mRNA in the human thyroid cell line HTori3 and in cultures of primary human thyroid follicular cells (TFCs). Cytokine mRNA levels were examined over a 24-h period, and the modulatory effects of exogenous IL-1α, interferon-γ (IFN-γ) and TSH investigated. Basal expression of IL-1α, IL-6 and IL-8 mRNA was detected in HTori3 and primary TFC cultures. Stimulation with IL-1 (10 U/ml) for 12 h produced an increase in the level of IL-1α mRNA in both primary TFC and HTori3 cultures. IL-6 and IL-8 mRNA levels were increased by the addition of IL-1 in both cell types, and this effect was detected throughout the 24-h time-course. IFN-γ (100 U/ml) had no significant effect on cytokine gene expression. A higher concentration of IFN-γ (500 U/ml) had no significant effect on the expression of IL-1α or IL-8 but produced an increase in the level of IL-6 mRNA in primary cultures and in HTori3 cells. Addition of TSH (1 mU/ml) produced an increase in the level of IL-1α mRNA in primary TFC and HTori3 cells, at 12 and 24 h. TSH had no significant effect on the expression of IL-6 or IL-8 mRNA. These results demonstrate that human TFCs constitutively express IL-1α, IL-6 and IL-8 mRNA and that this expression can be modulated by IL-1, IFN-γ and TSH.
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Online ISSN: 1479-6813
Print ISSN: 0952-5041
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